FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-58-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-58-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143460
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC4420.3080998187648125No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT4400.30670570193782243No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT4110.2864910079464659No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG4090.28509689111947584No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA4080.28439983270598074No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC4040.28161159905200056No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG3930.27394395650355496No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT3920.27324689809006No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT3920.27324689809006No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT3820.2662763139551094No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA3780.26348808030112925No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC3310.2307263348668618No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA3270.22793810121288163No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA3230.22514986755890143No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA3190.22236163390492122No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT3180.22166457549142618No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA2990.2084204656350202No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT2940.20493517356754495No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG2880.20075282308657463No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA2830.1972675310190994No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT2820.19657047260560434No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC2790.19447929736511918No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA2770.1930851805381291No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC2750.19169106371113898No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA2730.1902969468841489No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA2680.18681165481667364No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA2640.18402342116269343No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA2630.1833263627491984No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC2560.17844695385473303No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT2510.17496166178725778No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT2470.17217342813327757No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA2460.1714763697197825No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA2450.17077931130628746No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC2430.16938519447929737No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG2410.16799107765230725No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA2390.16659696082531716No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG2390.16659696082531716No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA2360.164505785584832No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT2360.164505785584832No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA2350.16380872717133696No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA2350.16380872717133696No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC2310.16102049351735676No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT2300.1603234351038617No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT2270.15823225986337655No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA2250.15683814303638646No Hit
GGGTAGTAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTC2240.1561410846228914No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT2230.15544402620939635No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC2220.1547469677959013No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT2210.15404990938240626No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA2190.15265579255541614No Hit
ATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTAAACATTGGCTG2190.15265579255541614No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT2190.15265579255541614No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG2120.14777638366095078No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2110.14707932524745573No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA2040.14219991635299037No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG2040.14219991635299037No Hit
GTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTTTGTTG2030.14150285793949532No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT2030.14150285793949532No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT2000.13941168269901016No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA2000.13941168269901016No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG1960.13662344904503No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC1940.13522933221803987No Hit
GTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAA1940.13522933221803987No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA1940.13522933221803987No Hit
GTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTA1920.13383521539104978No Hit
ATCATCATCTAACCAATCTTCTTCTTGCTCTTCTTCAGGTTGAAGAGCAG1900.13244109856405967No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA1900.13244109856405967No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC1900.13244109856405967No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT1890.13174404015056462No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA1880.13104698173706958No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT1880.13104698173706958No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT1870.13034992332357453No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA1870.13034992332357453No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA1860.12965286491007946No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA1830.12756168966959433No Hit
AGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAACCTCAA1830.12756168966959433No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA1830.12756168966959433No Hit
TCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGT1820.12686463125609926No Hit
GTTAGTAGCCTTATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGT1820.12686463125609926No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC1810.12616757284260421No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG1800.12547051442910914No Hit
GCCTTTGAGTGTGAAGGTATTGTTTGTTACCATCATATTAGGTGCAAGGG1780.12407639760211905No Hit
GTGTAGGTCAACCACGTTCCCGAAGGTGTGACTTCCATGCCAATGCGCGA1780.12407639760211905No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT1770.123379339188624No Hit
CTCTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTT1760.12268228077512895No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT1750.1219852223616339No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC1690.1178028718806636No Hit
GAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGG1680.11710581346716854No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA1670.1164087550536735No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT1670.1164087550536735No Hit
GTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGC1670.1164087550536735No Hit
ACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATAT1660.11571169664017844No Hit
GTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACA1650.1150146382266834No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC1640.11431757981318835No Hit
CTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTAGTAGGTTGTTCT1640.11431757981318835No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA1640.11431757981318835No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA1630.1136205213996933No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG1620.11292346298619825No Hit
GAATAGTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTT1610.11222640457270319No Hit
ATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAGAA1610.11222640457270319No Hit
GGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGATAAATT1600.11152934615920815No Hit
GATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGG1600.11152934615920815No Hit
ATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAA1600.11152934615920815No Hit
AAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTG1590.11083228774571309No Hit
TATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCT1570.10943817091872299No Hit
ATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATGTTTAG1550.1080440540917329No Hit
CTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTAAGTACT1550.1080440540917329No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA1550.1080440540917329No Hit
AAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAA1540.10734699567823784No Hit
GTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAA1530.1066499372647428No Hit
ATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGC1530.1066499372647428No Hit
ACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCAC1520.10595287885124774No Hit
CTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATT1520.10595287885124774No Hit
TTCATAAACAGTGCCAAAGATGTTAGTTAGCCACTGCGAAGTCAACTGAA1510.1052558204377527No Hit
CACCAGACTCATCAAATAAGTAGTATGTAGCCATACTCCACTCATCTAAA1510.1052558204377527No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA1500.10455876202425764No Hit
GTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTT1490.10386170361076257No Hit
ATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGAT1490.10386170361076257No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA1460.10177052837027742No Hit
GTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTAT1450.10107346995678237No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA1450.10107346995678237No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC1450.10107346995678237No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG1450.10107346995678237No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAA151.2278272E-4145.01
AGGCAGT100.0070845773145.05
TACCCTT100.0070845773145.07
AGCTGAA100.0070845773145.09
CAGTGTT550.0131.818189
AATATTG450.0128.888892
GAAGACA600.0120.8333364
AGACAGT600.0120.8333366
GAATATT550.0118.636361
GACAGTG650.0111.538477
GGGTAGT650.0111.538471
AAGACAG650.0111.538475
CTAGGGA203.8592357E-4108.759
AGTAGAG700.0103.571425
TCCCTAG352.7496026E-7103.571422
GAGGCGG650.0100.384629
GTAGAGG650.0100.384626
AGAGGCG650.0100.384628
TGAAGAC750.096.6666643
CAATGTG406.0906314E-790.6251
Produced by FastQC (version 0.11.5)