FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-57-B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-57-B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215497
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT7390.34292820781727823No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC6710.31137324417509293No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG6510.30209237251562665No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT6200.287707021443454No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA6180.28677893427750734No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC6120.2839946727796675No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT6090.28260254203074753No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA6000.2784261497839877No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT5700.2645048422947883No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT5640.26172058079694843No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG5470.2538318398864021No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC5070.2352700965674696No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT5030.2334139222355764No Hit
GTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGT4840.22459709415908344No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA4770.22134878907827024No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA4480.20789152517204415No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA4480.20789152517204415No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA4360.2023230021763644No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC4330.20093087142744448No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG4320.20046682784447115No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA4270.19814660992960462No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA4160.19304213051689814No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA4150.19257808693392484No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC4110.19072191260203158No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA3960.1837612588574319No Hit
CATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGATACTGGTTGCAAA3910.18144104094256533No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA3900.18097699735959202No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC3890.1805129537766187No Hit
CTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGAT3890.1805129537766187No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA3810.1768006051128322No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG3710.17216016928309907No Hit
CATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCT3700.17169612570012577No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA3680.17076803853417913No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG3550.16473547195552607No Hit
GTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTA3550.16473547195552607No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA3540.16427142837255274No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT3490.1619512104576862No Hit
GTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT3490.1619512104576862No Hit
CTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCACCTTCTT3410.15823886179389968No Hit
CATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACA3370.15638268746200643No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA3370.15638268746200643No Hit
ACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACT3340.15499055671308648No Hit
GGGTAGTAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTC3300.15313438238119323No Hit
CCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACC3210.14895799013443342No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG3190.1480299029684868No Hit
GTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAA3180.1475658593855135No Hit
TCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAAC3160.14663777221956686No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA3130.14524564147064692No Hit
CTCTAATTGAGGTTGAACCTCAACAATTGTTTGAATAGTAGTTGTCTGAT3130.14524564147064692No Hit
GTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAAT3130.14524564147064692No Hit
GTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATC3100.143853510721727No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA3090.14338946713875367No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC3080.14292542355578036No Hit
TCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGT3060.14199733638983375No Hit
GGTGACAATTTGTCCACCGACAATTTCACAAGCACAGGTTGAGATAAATT3060.14199733638983375No Hit
GTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTTTGTTG3050.14153329280686042No Hit
ATCATCATCTAACCAATCTTCTTCTTGCTCTTCTTCAGGTTGAAGAGCAG3020.1401411620579405No Hit
CATATTAGGTGCAAGGGCACAGTACTTTTCTGTGTCTTTGATTTCGAGCA3010.13967711847496717No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG3010.13967711847496717No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA2990.13874903130902053No Hit
GTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCA2980.13828498772604725No Hit
CAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATT2970.13782094414307391No Hit
TTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCAT2960.1373569005601006No Hit
ATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGT2960.1373569005601006No Hit
ATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTAAACATTGGCTG2930.13596476981118066No Hit
GCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAATTCAGA2920.13550072622820736No Hit
CCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAGTTCCAT2920.13550072622820736No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT2900.13457263906226072No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC2890.1341085954792874No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT2880.1336445518963141No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA2800.1299322032325276No Hit
CTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTA2790.12946815964955427No Hit
GTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCACCCACTTTAA2760.12807602890063435No Hit
CTCTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTT2750.12761198531766105No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC2740.1271479417346877No Hit
AAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAG2730.1266838981517144No Hit
GTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAA2690.12482772381982114No Hit
GTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGT2690.12482772381982114No Hit
GTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACA2670.12389963665387453No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA2660.12343559307090121No Hit
GTCCATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTA2630.12204346232198128No Hit
CTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAA2620.12157941873900796No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT2610.12111537515603465No Hit
CACCAGACTCATCAAATAAGTAGTATGTAGCCATACTCCACTCATCTAAA2590.12018728799008803No Hit
ATTAGTAGCTATGTAATCATCAGATTCAACTTGCATGGCATTGTTAGTAG2570.1192592008241414No Hit
ATGATAGAGTCAGCACACAAAGCCAAAAATTTATTTACAAGCTTAAAGAA2560.1187951572411681No Hit
CTTCTGTACCGAGTTCAACTGTATAGGCAGAGCACTTCTCATTAAGTACT2560.1187951572411681No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC2550.11833111365819478No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA2530.11740302649224815No Hit
GGCATGAGTAGGCCAGTTTCTTCTCTGGATTTAACACACTTTCTGTACAA2520.11693898290927483No Hit
GTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGC2520.11693898290927483No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT2510.11647493932630153No Hit
GATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGG2500.11601089574332822No Hit
CCTTAATTTCCTTTGCACAGGTGACAATTTGTCCACCGACAATTTCACAA2480.11508280857738158No Hit
GAATAGTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACCAACAGTT2470.11461876499440829No Hit
TTCATAAACAGTGCCAAAGATGTTAGTTAGCCACTGCGAAGTCAACTGAA2460.11415472141143497No Hit
AGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGAACCTCAA2430.11276259066251504No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC2410.1118345034965684No Hit
GGTGTAAGTAATTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGC2410.1118345034965684No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA2410.1118345034965684No Hit
GAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGG2400.11137045991359508No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA2400.11137045991359508No Hit
GTATACATTGTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAA2390.11090641633062179No Hit
TATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCT2390.11090641633062179No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC2390.11090641633062179No Hit
ACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATAT2380.11044237274764847No Hit
GTCAGTAAGTTTTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAA2360.10951428558170183No Hit
ACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAA2340.10858619841575522No Hit
GTTTCTCCCTCTAAGAAGATAATTTCTTTTGGGGCTTTTAGAGGCATGAG2340.10858619841575522No Hit
CTGGTGTACCAACCAATGGAGCTTCAACAGCTTCACTAGTAGGTTGTTCT2340.10858619841575522No Hit
TTCAGATACTGGTTGCAAAGTTTTTATGACAGCATCTGCCACAACACAGG2330.1081221548327819No Hit
GCCTTTGAGTGTGAAGGTATTGTTTGTTACCATCATATTAGGTGCAAGGG2290.10626598050088865No Hit
ATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATGTTTAG2290.10626598050088865No Hit
CTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATT2280.10580193691791533No Hit
ACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCACAATCAC2270.105337893334942No Hit
GTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTAT2230.10348171900304876No Hit
GTTAACACTTCTGTGGGAAGTGTTTCTCCCTCTAAGAAGATAATTTCTTT2230.10348171900304876No Hit
GCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACA2220.10301767542007546No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA2220.10301767542007546No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA2200.10208958825412882No Hit
ATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGC2200.10208958825412882No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA2190.1016255446711555No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC2190.1016255446711555No Hit
AATAAGTAGTATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGG2190.1016255446711555No Hit
CCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTAC2190.1016255446711555No Hit
CTTCTATCACAGTGTCATCACCAAAAGTAACCTTTGTTGGTGCACCGCCT2190.1016255446711555No Hit
GTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACC2170.10069745750520889No Hit
CACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAAC2170.10069745750520889No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGCC100.0070870286145.02
TGCGCAT100.0070870286145.04
GCGCATC100.0070870286145.05
TTGCGCA100.0070870286145.03
TTTGCGC151.2286767E-4145.02
GCTCAAT550.0131.818192
GAAGACA450.0128.888894
AAGACAG450.0128.888895
TCAATGT600.0120.8333364
CTGCCAT256.453276E-6116.03
GAATATT700.0113.9285661
AATATTG700.0113.9285662
TTGTCGT203.861896E-4108.752
CCTTACT203.861896E-4108.759
GACAGTG553.6379788E-12105.454557
AGACAGT553.6379788E-12105.454556
AGTAGAG700.0103.571425
TCCCTAG352.754332E-7103.571422
GTAGAGG650.0100.384616
TGCCATT301.5965987E-596.6666644
Produced by FastQC (version 0.11.5)