FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-57-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-57-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278771
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA11440.4103726714758709No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT10270.3684027391658386No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA8910.3196171768225533No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT6950.249308572268995No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT6200.22240476950615382No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA5980.21451298736238708No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG5900.2116432484010173No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG5840.20949094417999004No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA5550.19908814044502476No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT5550.19908814044502476No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC5340.19155507567142924No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC5170.18545688037851857No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG4910.17613022875406698No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC4840.17361920716286844No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC4690.1682384466103002No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTATGGATTTGTTTATGAGAATCT4690.1682384466103002No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA4610.1653687076489305No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC4370.1567594907648213No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC4120.1477915565105409No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG4100.14707412177019846No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA3950.14169336121763024No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG3940.14133464384745903No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC3890.13954105699660294No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG3810.13667131803523322No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC3770.13523644855454836No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC3680.13200799222300744No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3650.13093184011249376No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA3610.1294969706318089No Hit
CCCTTTACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA3600.1291382532616377No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC3550.12734466641078163No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT3470.1244749274494119No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA3470.1244749274494119No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT3440.12339877533889823No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT3430.12304005796872702No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT3420.12268134059855582No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA3370.12088775374769972No Hit
GTATACACCCCTCTTAGTGTCAATAAAGTCCAGTTGTTCGGACAAAGTGC3300.11837673215650121No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG3300.11837673215650121No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA3290.11801801478633No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT3250.11658314530564513No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT3140.11263725423376175No Hit
CCCTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA3100.11120238475307691No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT3070.11012623264256326No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT3070.11012623264256326No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT2980.1068977763110223No Hit
TAATTATACTGCGTGAGTGCACTAAGCATGCAGCCGAGTGACAGCCACAC2980.1068977763110223No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG2970.1065390589408511No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT2960.10618034157067986No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT2930.10510418946016623No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA2870.10295188523913894No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG2860.10259316786896773No Hit
GTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAA2830.10151701575845408No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC2830.10151701575845408No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA202.1278847E-6145.02
AACCCTA400.0145.05
CCGCTTG100.007088139145.0145
CCCACAG100.007088139145.07
TCGGACG100.007088139145.06
CTTTGAG100.007088139145.09
GACGGCT100.007088139145.09
CACAGCA100.007088139145.09
GGACGGC100.007088139145.08
CCCTTGT100.007088139145.01
TTAACCC400.0145.03
AGTGTAC100.007088139145.05
CGGACGG100.007088139145.07
CTTAACC450.0128.888892
TATAACC301.0995973E-7120.8333362
ACCCTTC203.8631007E-4108.757
CCTATAC600.0108.752
CCCTAAT553.6379788E-12105.4545447
CAAATGG352.7564784E-7103.571433
CCTAATT607.2759576E-1296.6666648
Produced by FastQC (version 0.11.5)