FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-56-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-56-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21443
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1700.7927995149932379No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1030.4803432355547265No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT910.42438091684932144No Hit
ACTCTGAGTTGTTGCAAAACTGAGAGTAAGACTACTGATGTGCACTTTAC790.3684185981439164No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT750.34976449190878145No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT690.3217833325560789No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG670.3124562794385114No Hit
CCCTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG660.3077927528797277No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC630.29380217320337637No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA600.2798115935270251No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT580.27048454040945763No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG570.2658210138506739No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG550.2564939607331064No Hit
CCCTTTACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA530.24716690761553886No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA460.2145222217040526No Hit
GTATAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCC450.20985869514526886No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT440.20519516858648507No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT420.19586811546891758No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG410.19120458891013384No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC410.19120458891013384No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG410.19120458891013384No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA400.1865410623513501No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG400.1865410623513501No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA390.18187753579256635No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT390.18187753579256635No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT390.18187753579256635No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA380.17721400923378258No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG370.17255048267499884No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT370.17255048267499884No Hit
CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG360.16788695611621507No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA360.16788695611621507No Hit
CTTGTAGATCTGTTCTCTAAACGAACTTATGGATTTGTTTATGAGAATCT360.16788695611621507No Hit
GTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGA360.16788695611621507No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC360.16788695611621507No Hit
GGGTAATTGAGTTCTGGTTGTAAGATTAACACACTGACTAGAGACTAGTG350.16322342955743133No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT340.15855990299864758No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC340.15855990299864758No Hit
GCTTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT330.15389637643986384No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT320.14923284988108007No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA310.14456932332229633No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT310.14456932332229633No Hit
ATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCG310.14456932332229633No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT310.14456932332229633No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG300.13990579676351256No Hit
ACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGT300.13990579676351256No Hit
GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT300.13990579676351256No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC290.13524227020472881No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT290.13524227020472881No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC290.13524227020472881No Hit
GAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACA290.13524227020472881No Hit
AAATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATT280.13057874364594507No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT280.13057874364594507No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC280.13057874364594507No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC270.1259152170871613No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT270.1259152170871613No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA270.1259152170871613No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT270.1259152170871613No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC270.1259152170871613No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC260.12125169052837756No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA260.12125169052837756No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG260.12125169052837756No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG260.12125169052837756No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA260.12125169052837756No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA260.12125169052837756No Hit
CTTTAGAGTCATTTTCTTTTGTAACATTTTTAGTCTTAGGGTCGTACATA250.1165881639695938No Hit
GACATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACA250.1165881639695938No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT250.1165881639695938No Hit
GTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAA250.1165881639695938No Hit
GCTTAGAATATATTTTAAAATAACCATCAATATTCTTAAACACAAATTCC250.1165881639695938No Hit
GGGTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTG250.1165881639695938No Hit
GTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGAC250.1165881639695938No Hit
CATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTG250.1165881639695938No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT240.11192463741081006No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG240.11192463741081006No Hit
ATTTTAGACTGTACAGTGGCTACTTTGATACAAGGTTTGCCACCAACACC240.11192463741081006No Hit
GTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGA240.11192463741081006No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT240.11192463741081006No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG240.11192463741081006No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA240.11192463741081006No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT230.1072611108520263No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC230.1072611108520263No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG230.1072611108520263No Hit
GGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATA230.1072611108520263No Hit
TAATTATACTGCGTGAGTGCACTAAGCATGCAGCCGAGTGACAGCCACAC230.1072611108520263No Hit
CTTTATAGCCACGGAACCTCCAAGAGCTAGCTTTTGTTGTATAAACCCAC230.1072611108520263No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC220.10259758429324253No Hit
GGTAAGAACAAGTCCTGAGTTGAATGTAAAACTGAGGATCTGAAAACTTT220.10259758429324253No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG220.10259758429324253No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA220.10259758429324253No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA220.10259758429324253No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA220.10259758429324253No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA220.10259758429324253No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC220.10259758429324253No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC220.10259758429324253No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC220.10259758429324253No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT220.10259758429324253No Hit
AGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTA220.10259758429324253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCTA151.2133832E-4145.05
CTTAACC151.2133832E-4145.02
GCTAGAC100.007042793144.999981
ACCTAGT100.007042793144.999988
CCCTTTA100.007042793144.999981
CCTAGTC100.007042793144.999989
GCTTTAC100.007042793144.999981
TAGACAC100.007042793144.999983
CCTAATT203.814003E-4108.749998
TTAACCC203.814003E-4108.749993
CCCTAAT203.814003E-4108.749997
ACAATAT203.814003E-4108.749998
ACCCTAA353.360838E-582.857146
GTTATGG350.003519009662.142857145
AGGTTTA501.9659227E-458.07
TGTCTTG400.005970606654.3749969
TAACCCT400.005970606654.3749964
TTTACCT400.005970606654.3749963
AAACTCT701.6404027E-551.7857136
CTCTGAA604.8366783E-448.3333329
Produced by FastQC (version 0.11.5)