FastQCFastQC Report
Thu 28 May 2020
000000000-J649D_l01_n01_CV-55-A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J649D_l01_n01_CV-55-A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156043
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA16121.0330485827624436No Hit
ATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGT8330.5338272142934961No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT5860.3755375120960248No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA5170.331318931320213No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG4940.3165794043949424No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG4920.3152977064014406No Hit
ACTCTGAGTTGTTGCAAAACTGAGAGTAAGACTACTGATGTGCACTTTAC4910.31465685740468974No Hit
ATGTAATTCAATTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATT4750.3044032734566754No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA3980.2550579007068564No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG3930.251853655723102No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT3730.23903667578808407No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC3650.2339098838140769No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT3610.2313464878270733No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC3570.22878309184006973No Hit
CCCTTAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCT3500.2242971488628135No Hit
GTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAG3480.22301545086931165No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA3420.21917035688880632No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT3410.21852950789205539No Hit
GTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGT3380.2166069609018027No Hit
GTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGA3220.20635337695378836No Hit
ATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATTCT3190.20443082996353568No Hit
GTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGA3150.20186743397653212No Hit
GCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGCAATAC3050.19545894400902317No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA2990.19161385002851777No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG2870.18392366206750704No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT2870.18392366206750704No Hit
GATTATAAGAGCCCACATGGAAATGGCTTGATCTAAAGCATTACCATAAT2860.18328281307075614No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG2830.18136026608050346No Hit
ATCTAAAGCATTACCATAATAAACTTTATAAACGAGTGTCAAGACATTCA2780.17815602109674897No Hit
GTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTC2740.17559262510974538No Hit
CTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCAT2730.1749517761129945No Hit
GTAATAAACACCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTT2720.1743109271162436No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC2650.16982498413898733No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA2650.16982498413898733No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA2630.16854328614548553No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC2630.16854328614548553No Hit
CTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTT2610.16726158815198375No Hit
CCCAAAAATGGATCATTACAAAATTGAAATTCACAGACTTTAATAACAAC2590.16597989015848194No Hit
GGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGC2530.16213479617797658No Hit
ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG2510.1608530981844748No Hit
GCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATT2500.1602122491877239No Hit
ATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCG2490.159571400190973No Hit
ACATAACACAGTCTTTTAGCTTAAAACCAGACAAACTAGTATCAACCATA2460.1576488532007203No Hit
GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT2450.1570080042039694No Hit
GGCATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAA2440.15636715520721853No Hit
GATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAAC2440.15636715520721853No Hit
GTAATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAG2430.15572630621046762No Hit
TCTTAGTACCATTGGTCCCAGAGACATGTATAGCATGGAACCAAGTAACA2420.15508545721371672No Hit
GCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCT2380.15252206122671313No Hit
GTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAA2330.14931781624295867No Hit
GTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCAT2310.1480361182494569No Hit
ACCCAATATGGTACGTCCATTCATACCATTTTGCAGTAATTCTTTTAATG2280.14611357125920418No Hit
CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG2270.1454727222624533No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC2190.14034593028844614No Hit
CTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGA2180.13970508129169523No Hit
AGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTA2180.13970508129169523No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT2170.13906423229494433No Hit
ATTCAAAAGTGCAATTATTCGCACTAGAATAAACTCTGAACTCACTTTCC2160.13842338329819345No Hit
GTCTAAAGTAGCGGTTGAGTAAACAAAAGAGGCCAAAGTAACAAGTACAA2150.13778253430144255No Hit
CTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTTG2140.13714168530469167No Hit
GTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATT2130.13650083630794077No Hit
CATAAAAACAATACCTCTGGCCAAAAACATGACAGTTGTAACTACACCTG2120.13585998731118987No Hit
ATGTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAAT2090.13393744032093718No Hit
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGAT2090.13393744032093718No Hit
GGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG2070.13265574232743538No Hit
AAATTAATAGGCGTGTGCTTAGAATATATTTTAAAATAACCATCAATATT2040.1307331953371827No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC2020.12945149734368092No Hit
GTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGA2010.12881064834693No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT2000.1281697993501791No Hit
GATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACA2000.1281697993501791No Hit
CAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCC2000.1281697993501791No Hit
CATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAA1990.1275289503534282No Hit
CCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAAGGTCCATAAGAAAA1980.12688810135667733No Hit
GTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTT1960.12560640336317552No Hit
ATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCT1960.12560640336317552No Hit
CATGTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAG1950.12496555436642463No Hit
CCCTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAG1930.12368385637292285No Hit
TCAATATTCTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTT1900.12176130938267016No Hit
GTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAAAC1900.12176130938267016No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA1880.12047961138916838No Hit
GGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCAT1880.12047961138916838No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG1880.12047961138916838No Hit
AATTATAAGTCTACCTTTACTAAGAAGAGATAAAATCATATCATTGATTT1870.11983876239241746No Hit
GTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCT1870.11983876239241746No Hit
GTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTT1870.11983876239241746No Hit
ATATAGACCATATTAAAATAAGCTACAGTGGCAAGAGAAGGTAACAAAAA1870.11983876239241746No Hit
GTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACT1870.11983876239241746No Hit
AATAAACACCACGTGTGAAAGAATTAGTGTATGCAGGGGGTAATTGAGTT1860.11919791339566657No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA1790.1147119704184103No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA1780.11407112142165941No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC1740.11150772543465583No Hit
ATTTTAGACTGTACAGTGGCTACTTTGATACAAGGTTTGCCACCAACACC1740.11150772543465583No Hit
ACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGA1730.11086687643790494No Hit
GCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCCTGAGT1730.11086687643790494No Hit
GTGTATTACCAGTTATGAAGAAAATAGGGCAATACTCAACACACATAAAA1700.10894432944765224No Hit
ATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCA1700.10894432944765224No Hit
GGGTAATTGAGTTCTGGTTGTAAGATTAACACACTGACTAGAGACTAGTG1690.10830348045090135No Hit
GACTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGG1690.10830348045090135No Hit
GTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCT1680.10766263145415046No Hit
AGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTG1670.10702178245739957No Hit
GTCATAATCTATGTTAAAACCAACACTACCACATGAACCATTAAGGAATG1670.10702178245739957No Hit
CTTAAACACAAATTCCCTAAGATTTTTGAAATTACCCTGTTTTCCTTCAA1660.10638093346064866No Hit
GTAGTATCTTTAGCTAAGAGAATGTCATTGTGTAACTGGACACATTGAGC1640.10509923546714688No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA1630.10445838647039599No Hit
GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT1630.10445838647039599No Hit
ATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCT1620.10381753747364508No Hit
CTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTAT1600.10253583948014329No Hit
CAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAAT1570.10061329248989061No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA100.007085167145.01
GTCAATG100.007085167145.09
AACCCTA351.891749E-9124.2857065
CTTAACC351.891749E-9124.2857062
TTAACCC351.891749E-9124.2857063
CCTATAC203.859876E-4108.752
CCTTATA404.789399E-9108.752
CCCTTTA352.7507485E-7103.571421
AAATTAG2800.095.8035665
CCTAATT502.2560926E-887.08
TAACCTC259.371796E-487.05
CCCTTAT502.2560926E-887.01
CCCTAAT502.2560926E-887.07
ATAGGCG851.8189894E-1276.764715
ATAAATT3600.076.527783
GGCGTGT903.6379788E-1272.58
TTTTATA406.645587E-572.52
TAATAGG955.456968E-1268.684213
GCGTGTG1051.6370905E-1162.1428579
GTATAAA5850.058.2478681
Produced by FastQC (version 0.11.5)