FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185916
Sequences flagged as poor quality0
Sequence length251
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111165.979044299576152No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT18651.0031412035542933No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.4093246412358269No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6550.3523096452161191No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.3254157791690871No Hit
NTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.23827965317670344No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.1861055530454614No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2550.13715871683986317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA4100.0200.617341
GCGATAC4250.0193.212252
CGACAGC5200.0183.969538
CGATACG4650.0176.542533
TACGACA5200.0155.008016
GATACGA5550.0152.337554
ATACGAC5450.0150.585975
CCGCTAT1400.0126.543241
GCTATCG1550.0114.073643
CGCTATC1700.0104.037382
TCGACAG1900.099.595947
CTATCGA2000.094.6425554
GACAGCA10300.092.722949
ACGACAG8500.092.018837
TATCGAC2250.084.173695
TCATGGG4350.041.7429725-29
GGGACTG4050.040.37143330-34
GCACGAC4100.040.35729235-39
AGTCGAC3500.040.16695440-44
CTGCACG4050.039.1480630-34
Produced by FastQC (version 0.11.5)