FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n02_4.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n02_4.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105747
Sequences flagged as poor quality0
Sequence length251
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG707.895408E-570.01
TCGGGAG801.528943E-461.2499963
CGGGAGA852.0633105E-457.6470574
ATCGGGA852.0633105E-457.6470572
GAGGGGC902.7365997E-454.4444479
CTCTAGT750.00910890549.0000044
GCGTCGG1506.860098E-540.833336245
CGGTGGG2054.3021207E-429.878048245
GGAGAGG1800.00823955227.2222236
GGGGGGC1204.7039597E-516.33333440-44
CGGGGGG1256.6694425E-515.6835-39
GGGGGCG1156.3011015E-414.91304440-44
TGTGTTG1100.00806770713.363637240-244
GGGGCGG1400.002777987912.25000110-14
GGGGGGG3302.5700501E-811.13636340-44
GGGGAAG2002.8214577E-411.02520-24
GGAGAAG2750.00517233748.018182235-239
AGGAGAG2800.0060808447.8750005240-244
GGAGAGA3300.00316647677.424242240-244
GAGAGAG6600.00288281365.568182245
Produced by FastQC (version 0.11.5)