FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n02_3.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n02_3.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365932
Sequences flagged as poor quality0
Sequence length251
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG1506.8832676E-540.8435131
TCGGGAG1601.00678866E-438.29083
GTAGGGG3353.0072079E-729.25303245
ATCGGGA2500.001376006924.506112
TCTCGGG7201.5097612E-920.416176245
ACCCCAA3500.00966424117.5043622
TCGCCGG8902.0891093E-816.516457245
AGAGCGG5655.5108557E-415.176628245
GCGTCGG5300.006310090413.867592245
AAAGAGG26650.011.9509325245
GGTGGTG7200.003427597711.909436245
CCCGTGG1450.003619167311.827302100-104
AGAGAGG15751.6137346E-710.888628245
GAGATAA13250.00370012718.320555245
GAAGAGG17708.201851E-58.304886245
GAGCGAG2750.00518579648.01799240-244
ATTGCCA6200.00131826567.9041169
CCCACTT2900.0083469757.60326625-29
TGTGAGT2950.0097228177.474397235-239
AATAGAA26451.7079191E-46.4837766245
Produced by FastQC (version 0.11.5)