FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n02_2.5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n02_2.5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324209
Sequences flagged as poor quality0
Sequence length251
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA1552.3683242E-855.345024
GATCGGG1603.042078E-853.615491
CGCGAAC700.006940657352.5131958
TCGGGAG1501.1984284E-649.0198753
GAGGGGC1751.7068074E-435.0033959
TCGCCGG6600.029.695269245
ATCGGGA2503.9870232E-529.4119222
TCGTGTG2155.713088E-428.48674245
CCACAGG1800.00825417627.229066
GCGTCGG4051.5757214E-624.196146245
GTAGGGG3101.7275791E-423.708319245
GGAGAGC2650.001931118223.1190136
GAGCGGC2750.002397153522.2748879
GAGAGCG3200.00576250719.1454357
TCTCGGG5201.3737159E-518.845074245
TCGGTCG700.00969523117.50713
GGGAGAG5904.0567087E-516.6143445
GTAGATT5253.1517624E-416.332397245
CGGGGGG2454.9058144E-913.99919735-39
GGGGGGC2551.2462078E-712.48948140-44
Produced by FastQC (version 0.11.5)