FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n02_2.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n02_2.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176435
Sequences flagged as poor quality0
Sequence length251
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC1401.8189894E-1278.759
TCTCGGG3250.045.230766245
AGAGGGG3707.1320574E-726.4864868
GCGTCGG1850.00943418826.486486245
AGGGGCG858.305324E-825.94117710-14
CGGTGGG3151.9198813E-423.333334245
GGGAGAG3756.2347157E-419.65
GGCGGGG756.2618987E-419.610-14
GGGGGGC1601.12397174E-716.8437540-44
CGGGGGG1602.075547E-615.312535-39
GGGGGCG1452.3605446E-413.51724240-44
TCGGGGG2805.9156577E-59.62500135-39
GGGGAAG3751.7679431E-69.14666720-24
GGAGGGG3953.3504577E-58.06329215-19
GGGGGGG6602.0970947E-77.053030540-44
GTATGAT3600.00739104776.8055553230-234
AGGGGAA4400.00100809566.68181820-24
AAGAGGG7808.1619725E-46.2820517
CCCCCCC5300.00123409936.009434155-159
GGGAAGA6352.8504737E-45.787401720-24
Produced by FastQC (version 0.11.5)