FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n02_1.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n02_1.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444228
Sequences flagged as poor quality0
Sequence length251
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGG3300.006897320518.560392245
AAACCGG3350.00751260718.2874891
GCGTCGG6703.2424032E-718.283373245
TATGCTG5200.005559573414.13604457
TCTCGGG8704.9443206E-614.080298245
GTATTGG5300.006302963513.8708882
CGGTGGG9601.3655566E-512.76027245
CCCCCGT1657.028355E-411.878652135-139
CTCCCGG1450.003619245311.8274570-74
ATAGAGG8509.003621E-411.52928245
CTTGATG7650.005383110611.2115451
AGAGTGG10750.0063261229.116175245
GAAGAGG22452.4039946E-78.730412245
GTGAGTA6959.6505997E-47.050278245
GTGTGAG5300.0081016336.933882245
AAAGAGG28105.3070282E-56.53907245
TGTAGTG4200.0045591436.416593240-244
GTGAGAG10203.6038546E-75.5244465240-244
Produced by FastQC (version 0.11.5)