FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n01_4.5-wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n01_4.5-wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5119
Sequences flagged as poor quality0
Sequence length251
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1232.4028130494237154No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC60.1172103926548154TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTACA100.0014335114245.00002245
TTTTTTT1208.3056773E-414.29166760-64
GGGGGGG21150.05.7919624
Produced by FastQC (version 0.11.5)