FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n01_4.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n01_4.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105747
Sequences flagged as poor quality0
Sequence length251
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC4620.4368918267184885TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1670.15792410186577396No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCCG600.003767599161.259
GGTTCGG600.003767599161.257
TTCGGTC750.00910890549.0000049
ATTTCAG2257.416738E-427.2222211
ATAGAGA4200.00454269356.4166665240-244
AAAGGAG4800.0026833676.125220-224
Produced by FastQC (version 0.11.5)