FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n01_3.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n01_3.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365932
Sequences flagged as poor quality0
Sequence length251
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4540.12406676650306614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCACC3050.00437299920.0819669
CCTGATT3100.00480440119.7580643
TGTATTC5907.6514E-414.5338985
AGAGAGG10350.00462615549.468598245
AAGATTT14100.00647204657.8191494
AATCACC3801.8810877E-47.73684230-34
ATGTGAG4900.00339199447.5245
CCCCCCC3550.00646331866.90140845-49
TGAGTAG4550.00147590526.4615383240-244
GAGGTAG4800.0026935446.125240-244
ATGAGTA7153.8337694E-55.825175240-244
GAAGGGA6350.00178402145.401575240-244
AAAAGGA31950.00191570645.367762245
GTAAGAG6500.002392495.276923235-239
AGATAGT8407.707651E-55.25230-234
AGAAGTG8601.1162049E-45.127907220-224
Produced by FastQC (version 0.11.5)