FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n01_2.5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n01_2.5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324209
Sequences flagged as poor quality0
Sequence length251
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4800.14805264505303678No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4680.14435132892671085TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAGTT9200.001741760410.6521745
TAATGTG4900.00339132697.50000059
GGGGGGG77300.05.54657179
GTGAGAA6450.00217095445.3178296235-239
Produced by FastQC (version 0.11.5)