FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n01_2.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n01_2.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176435
Sequences flagged as poor quality0
Sequence length251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3880.21991101538810326No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3790.21480998668064727TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC750.00911894848.9999965
TGCTCTG1500.003385278232.6666642
CGGGACT600.003978300420.41666655-59
TACGCGT650.0063199118.84615590-94
CCGTTAG700.00968863617.50000225-29
CCTACAG1050.00593378914.050-54
AAGAATA15501.7722795E-48.693548245
ACTTTGT2850.00713568137.7368417115-119
AGGATGT2950.0097108227.474576215-219
GAGTAGA3450.0048921937.101449225-229
AGAGTAT3600.00739104776.8055553240-244
AGAGAGT3700.0096277986.6216216240-244
ATGCCGG4250.00514868836.3411765200-204
GAAGGTA4300.00581514936.2674417240-244
CGGCTTC5600.00238252435.6875005205-209
Produced by FastQC (version 0.11.5)