FastQCFastQC Report
Sat 14 Nov 2020
000000000-J63M6_l01_n01_1.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J63M6_l01_n01_1.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444228
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4950.1114292660525676No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCA2950.003606447320.7627129
GTAAGCT3450.0089114917.7536222
TGCGTCC2550.00262307598.64705935-39
CGGGAGC2600.00312719828.48076965-69
GAAGAGG16303.081617E-48.2668705245
GCTCGCT3250.0027353467.538461720-24
AGGGAGG8005.755939E-66.1256
GAGTGTA5250.00110375036.066667225-229
AGAGAGA28654.8004632E-45.986038245
CTCCTCA5500.00192660545.79090920-24
GTAAGAG9553.5285975E-65.3874345235-239
AAGATAA32600.00247141675.260736245
GGTGTAT6150.00720555.1788616220-224
Produced by FastQC (version 0.11.5)