FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286866
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.5141773510977251No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC3830.13351181387825675No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3580.1247969435206682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT900.096.6666645
CCTAGGT1100.072.54
CTACCGT300.001934330272.56
TACCGTG300.001934330272.57
TCTACCG350.003563911762.1428575
TAGGTAG1300.061.3461536
GCACGCA1450.055.02
CTAGGTA1700.046.9117665
ACGCACT1950.044.6153874
AGCACGC1851.8189894E-1243.108111
GCCCAAG700.001055380241.428571
CACGCAC2100.041.428573
GGCTTAC700.001055380241.428577
TCCTAGG1953.6379788E-1240.8974383
GCAGGTA1451.9195795E-840.09
ACCGTGT750.00148162138.6666688
CCTAGCA1354.954909E-737.5925942
AGGTAGT2151.2732926E-1137.093027
CCCTAGC1458.6697037E-735.01
ACACGAG850.002738741734.117651
Produced by FastQC (version 0.11.5)