FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_E9_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_E9_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117963
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA3170.2687283300695981No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2780.23566711596008919No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2760.23397166908267847No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC2380.2017581784118749No Hit
GATCTGGACGTAGCCTTGGGCAGACCAAAATGCACGGGGAAAATACCCTC2230.18904232683129457No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2160.18310826276035705No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG2160.18310826276035705No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2040.17293558149589278No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT2030.17208785805718743No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC2020.17124013461848206No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA2020.17124013461848206No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1970.16700151742495528No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA1860.15767655959919635No Hit
GCATTATTGTCAGGGCCATCAACTCCGATATATGTCCATTCCTTACCATC1720.14580843145732136No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC1680.14241753770249993No Hit
GCTTGTTTCTGTCTCCTCTTGTTCCATTGTAGTATCCCCCTGGTTGGGCT1680.14241753770249993No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT1650.13987436738638387No Hit
CCATTGGATTGTGCAAACGCATCAAATGTTCAGGCTGTGAACCGTTCTGC1630.13817892050897315No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA1600.1356357501928571No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTACTTTGAGCAATG1590.13478802675415172No Hit
CCATCATACTTGACATACAGTCCCATCCCCATCCTTTCAGTTTTAGACAT1550.1313971329993303No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA1520.12885396268321422No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT1520.12885396268321422No Hit
ATCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAA1500.1271585158058035No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT1480.1254630689283928No Hit
CTCTTAAGGTCTGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTA1460.1237676220509821No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT1430.12122445173486601No Hit
GTCCCATCCCCATCCTTTCAGTTTTAGACATCGTTCGAGAGTACCACCTT1420.12037672829616065No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA1410.11952900485745531No Hit
GTCCTGAGCCCATTAAACAGTAAATGGCTGTTGCTGCTGAGTGCCAAGTC1400.11868128141874995No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA1380.11698583454133923No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT1380.11698583454133923No Hit
GGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGG1370.11613811110263386No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG1350.11444266422522317No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGTTTCCATTGGTAATGTTAGGGCA1350.11444266422522317No Hit
GTTTAGAACAGACTCAACCATTCCTCCATTACAGAGCCATGTCAACACCT1330.11274721734781244No Hit
GTCCAGATCTGTGCAGTTGAGGCATTTTGGGCATAGTTTCCCCCTGGTTT1320.11189949390910708No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA1300.11020404703169638No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT1300.11020404703169638No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA1300.11020404703169638No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT1280.10850860015428566No Hit
CTCCTAATTAGCCCTCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC1270.1076608767155803No Hit
GTATGATGGAGACCCATGGGCTGACAGTGATGCCCTAGCTTTTAGTGGAG1270.1076608767155803No Hit
GTGTAACTGTTATCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAA1210.10257453608334816No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG1210.10257453608334816No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG1210.10257453608334816No Hit
GCTCTATGGGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAG1200.10172681264464283No Hit
ATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAGGAGTA1200.10172681264464283No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT1180.1000313657672321No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCTT100.007082991145.05
TCTACTA100.007082991145.05
ACGGAAT100.007082991145.04
GAAACAC306.4574124E-10145.04
AAACACG306.4574124E-10145.05
AGGATAG100.007082991145.03
ACGAGCC100.007082991145.04
CGGAGCA151.2272777E-4145.05
GTAACAA1100.0112.045454
ACACGTG203.857515E-4108.757
AAGCGGA203.857515E-4108.752
GCGGAGC203.857515E-4108.754
AACACGT203.857515E-4108.756
TAGAAAC501.9281288E-10101.52
TAACAAG1350.091.2962955
CACGCAC259.3660865E-487.03
GCACGCA259.3660865E-487.02
AGCGGAG259.3660865E-487.03
ACGTGCA502.2511813E-887.02
AGCACGC259.3660865E-487.01
Produced by FastQC (version 0.11.5)