FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_E6_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_E6_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162427
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4650.28628245304044275No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT3640.22410067291767996No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC3470.21363443269899707No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3410.20994046556299137No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3110.19147062988296282No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3050.1877766627469571No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2870.17669476133893997No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA2560.1576092644695771No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC2150.13236715570687138No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC2100.12928884976019997No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA2060.12682620500286282No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC1960.12066959310951997No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC1950.12005393192018568No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC1950.12005393192018568No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT1950.12005393192018568No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA1900.11697562597351426No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG1860.11451298121617712No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT1770.10897203051216856No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC1760.10835636932283427No Hit
CTCCTAGACTCGGCACTAAACCCCTCCAATTGGGCATTTCCAAATACATA1750.10774070813349998No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC1750.10774070813349998No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT1730.1065093857548314No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT1730.1065093857548314No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA1720.10589372456549712No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT1670.10281541861882569No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT1650.10158409624015713No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAA100.0070854323145.000025
ACACGTG100.0070854323145.000027
TTAGAGA100.0070854323145.000024
GAAACAC202.12518E-6145.000024
AAACACG202.12518E-6145.000025
AACACGT100.0070854323145.000026
CACGCAC500.0145.03
GCACGCA500.0145.02
GTGTCAT151.2281237E-4145.06
AGCACGC500.0145.01
ACGCACT600.0120.8333364
TAGAAAC256.448452E-6116.02
ACGTGCA256.448452E-6116.02
CGCACTT650.0111.538475
CGTGCCT203.860164E-4108.750013
ACGTGCC203.860164E-4108.750012
CACGTGC501.9463187E-10101.51
GAAGCGG259.3724934E-487.01
GCACTTT950.083.947376
GGTATGC554.0463165E-665.9090968
Produced by FastQC (version 0.11.5)