FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_E5_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_E5_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170391
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4650.2729017377678398No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT3510.2059967956054017No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT3160.18545580459061803No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT3120.18310826276035705No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT2830.1660885844909649No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2790.1637410426607039No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2710.15904595900018192No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2610.15317710442452948No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC2530.1484820207640075No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2520.14789513530644224No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2470.14496070801861602No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2450.14378693710348553No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2100.12324594608870186No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC2040.11972463334331039No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT1990.11679020605548415No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT1980.11620332059791891No Hit
GATCTATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGC1920.11268200785252741No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG1920.11268200785252741No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT1910.11209512239496217No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA1900.11150823693739692No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1850.1085738096495707No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC1830.1074000387344402No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT1770.10387872598904872No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC1740.10211806961635299No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT1740.10211806961635299No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA1710.10035741324365723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCA400.0145.02
TGTCGGT100.0070857364145.02
GTAACAA1650.0123.0303044
CACGTGC650.0122.692311
CACGCAC301.0977419E-7120.8333363
GCACGCA301.0977419E-7120.8333362
AGCACGC352.75184E-7103.571431
TAGAAAC554.129106E-1092.272722
ACAACAG406.095579E-790.6251
CGTGCCT259.3732873E-487.03
TGAACTA259.3732873E-487.0145
ACGTGCC259.3732873E-487.02
AAACACG502.2573658E-887.05
GAAACAC554.3724867E-879.0909044
TAACAAG2750.073.8181845
TAGTAAC2950.073.728822
CAGCCTT300.001932977172.55
ACGCACT502.298024E-672.54
GAGCATC406.647343E-572.57
GCAGAGC1450.070.03
Produced by FastQC (version 0.11.5)