FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_E4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_E4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176908
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG9380.5302190969317385No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC8640.4883894453614308No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA6050.34198566486535376No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG5670.32050557351843895No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4830.27302326633052204No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC4640.26228322065706466No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA4540.2566305650394556No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA4470.25267370610712914No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3960.22384516245732244No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3800.21480091346914781No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA3380.19105975987518936No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT3340.18879869762814572No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC3290.18597236981934112No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA3200.1808849797634929No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT3150.1780586519546883No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC3060.17297126189884007No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA3040.17184073077531825No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA2950.16675334071947No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA2890.16336174734890452No Hit
GATATAGCCACAGCCAAGGCATGCTGGCGGCCGCCAGAAGCTGGAAGAGG2820.1594048884165781No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG2750.15544802948425168No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2710.15318696723720804No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA2710.15318696723720804No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2630.14866484274312072No Hit
CCTTATAAGAGACAAGCAGAGGGAGATGTAACATGCAGGCACACAACAGG2430.13735953150790242No Hit
ATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCTTGCTT2350.1328374070138151No Hit
AAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA2330.13170687589029326No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT2310.13057634476677143No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA2280.1288805480814887No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT2220.1254889547109232No Hit
GAAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2220.1254889547109232No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA2180.12322789246387952No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT2170.12266262690211861No Hit
GTCCTACTGCAGCCTGTTGCCAAGGAGCTCCCCACAGACATGGAGCTCTC2110.11927103353155312No Hit
GTAGTAGGTTGAATAATTCCCTGCCCTCTCCCACTCAGTCCTCAAAGATA2100.11870576796979221No Hit
CACTAAAGGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCAC2060.11644470572274854No Hit
CCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGTGACAGGC2050.11587944016098763No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT1970.11135731566690031No Hit
GCATAACTCAGTGTTACAAGCTGGGCTTTAAAGCCAGGTTCTCTGGTTTA1940.10966151898161756No Hit
CTCCCACTCAGTCCTCAAAGATAGCAGGCCCTAGTCCTGGAGGCTGTAAA1920.10853098785809573No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC1900.10740045673457391No Hit
CCCTCTCTACCTTGCATGGTCTCTGCCCAGGACGCACCTCTGCCTAAGGG1900.10740045673457391No Hit
GTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGCTCACTCTCAC1890.10683519117281298No Hit
TCCCATGGCTGTAGAATCCATCAAGGCTTTGCCGATCTCTTGACCTGTTT1890.10683519117281298No Hit
CCCTTAGGCAGAGGTGCGTCCTGGGCAGAGACCATGCAAGGTAGAGAGGG1880.10626992561105207No Hit
GTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCTTGGAAGCCAGA1880.10626992561105207No Hit
CTCCTGAGGAAGCCACGGGGAGCCTTGCAGGATGTTTTGCTGGAAGGGAG1870.10570466004929116No Hit
GGGCAGAGAGTGTACCTCAAAACAAGGTGTACTTAAAAGAGAATCTGGGA1870.10570466004929116No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT1850.10457412892576932No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT1840.10400886336400841No Hit
GCCTCTTACAGTGCATTAGTTTCTGTAGCTGCTGTAACAGGGTACCACAA1820.10287833224048658No Hit
AGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA1800.10174780111696476No Hit
CACTAAAGATAGTTTATGCATAAGGGTCCTATGAGGTTTAGCTCAGAGCC1800.10174780111696476No Hit
GTCCAGCCCTGAGTTGGGTCCTCTCACTAAAGGACTAGAGAGTGCTTTTA1790.10118253555520385No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT1790.10118253555520385No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCT100.0070859645145.04
AGCACGC2800.0142.410721
ACGCACT2950.0135.16954
GCACGCA2950.0135.16952
TAGTAAC1800.0132.916672
CACGCAC3000.0132.916663
CGCACTT3050.0130.73775
CCCCGTT301.0979238E-7120.8333369
GCACTTC2150.0111.279076
GCACTTG1750.0103.571436
AGTAACA2300.0100.869563
CACTTGA1750.099.4285667
GTAGTAA2350.098.7234041
GCCCCCG451.0864824E-896.6666647
TAACAAG2400.096.6666645
CACTTCG301.5958043E-596.6666647
GAGCGAT502.2579115E-887.07
CCCCCGT502.2579115E-887.08
GTAACAA2700.085.925934
GAAGCGG1700.085.294111
Produced by FastQC (version 0.11.5)