FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_E3_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_E3_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218008
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG23471.0765659975780706No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC19690.9031778650324759No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA18800.862353675094492No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA18640.8550144948809217No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC18050.8279512678433819No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG16690.7655682360280356No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA16520.7577703570511174No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT12590.5775017430553008No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGG10160.46603794356170414No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT9510.43622252394407546No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA8500.38989394884591394No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC8300.38071997357895127No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG6810.31237385784007926No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA6740.3091629664966423No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC6690.30686947267990167No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA6600.30274118380976844No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC6010.27567795677222856No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG5820.26696268026861397No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG5560.2550365124215625No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG5310.24356904333785917No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA5150.236229863124289No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5010.22980808043741516No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT4960.2275145866206745No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA4770.21879931011705994No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG4760.21834061135371177No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4690.21512972001027486No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA4630.21237752743018604No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC4500.2064144435066603No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT4420.20274485339987522No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4400.20182745587317896No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG4360.19999266081978645No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT4280.19632307071300137No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA4230.19402957689626069No Hit
CCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGT3780.17338813254559465No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3750.17201203625555025No Hit
CTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3750.17201203625555025No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC3720.17063593996550586No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTC3610.16559025356867638No Hit
GTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCTAAGAG3480.15962716964515064No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC3460.15870977211845438No Hit
ATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT3390.15549888077501742No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA3360.15412278448497305No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC3330.15274668819492862No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG3300.15137059190488422No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3280.15045319437818797No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3260.14953579685149168No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3200.14678360427140288No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATG3200.14678360427140288No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA3170.14540750798135849No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT3140.1440314116913141No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT3090.1417379178745734No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATT3080.14127921911122526No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT2960.13577483395104767No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2900.13302264137095887No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT2830.12981175002752193No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG2740.1256834611573887No Hit
GTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2720.12476606363069245No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2710.12430736486734432No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2690.12338996734064804No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAA2620.12017907599721112No Hit
GCTTCAACCCGATGATTGTCGAACTTGCAGAAAAAGCAATGAAAGAGTAT2620.12017907599721112No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC2550.11696818465377418No Hit
CTTAGGGACAAACTCGAACCTGGGACCTTTGATCTTGGGGGGCTATACGA2520.11559208836372976No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2460.11283989578364097No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCACT2440.11192249825694471No Hit
GAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT2420.11100510073024843No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC2400.11008770320355217No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT2390.10962900444020401No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCC2360.10825290815015963No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2310.10595941433341896No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2290.10504201680672269No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2270.10412461928002642No Hit
ATGCTAAGTCAGTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGA2270.10412461928002642No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA2260.10366592051667829No Hit
CCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGCTCCTTGTTGT2240.10274852298998202No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2220.10183112546328577No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2220.10183112546328577No Hit
GCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGG2200.10091372793658948No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT203.8619587E-4108.751
GAGCGAA1250.098.61
TATAGTC259.376833E-487.07
GTATAAT259.376833E-487.03
CAAAAGC8850.082.740114
AAGCAGG11500.073.760877
GCAGGGT300.001933705172.59
AGCAGGT10100.069.628718
AAACTAT4200.069.0476156
TAGAAAC3400.068.23532
TAAACTA4450.065.168535
AAAGCAG13150.064.505716
GTAGAAA3700.062.7026981
GCAGGTA5800.062.5000049
GTGGGCT350.003562763862.142857145
AGCTTAT350.003562763862.1428571
TATATAT350.003562763862.1428575
ATATAGT350.003562763862.1428576
TAGGGAG350.003562763862.1428573
ATTATCA350.003562763862.1428573
Produced by FastQC (version 0.11.5)