FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_E1_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_E1_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193901
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG100.0070864856145.01
AGCGAAA950.083.947371
CAGTACG406.649659E-572.59
CGAAAGC1300.061.3461533
TGACCGA502.006187E-458.06
GGGGTAA651.0903417E-555.769231
CCGAGCC553.2125242E-452.7272729
ATGACCG553.2125242E-452.7272725
GTAGTGG553.2125242E-452.7272726
GCGAAAG1550.051.451612
CTATAAG604.935159E-448.3333321
GACCGAG657.321905E-444.6153837
GTAATCG657.321905E-444.6153838
TATTAGG657.321905E-444.6153832
TATAGAG657.321905E-444.6153832
GAGCAAA4200.041.428571
ACCGAGC907.460113E-540.277788
GTCTATA907.460113E-540.277781
TAATCGA750.00148043638.6666649
GCAAAAG4700.037.021283
Produced by FastQC (version 0.11.5)