FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_E12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_E12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184698
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT6730.3643786072399268No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT4250.2301053611842034No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA3190.1727143769829668No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3160.17109010384519593No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA3050.1651344356733695No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC2890.1564716456052583No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2840.15376452370897356No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC2700.1461845823993763No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2590.14022891422754985No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA2590.14022891422754985No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT2520.1364389435727512No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT2470.13373182167646644No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT2430.13156612415943866No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT2400.1299418510216678No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT2380.12885900226315392No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2370.12831757788389694No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC2350.12723472912538306No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC2290.12398618284984136No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT2190.11857193905727187No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT2180.11803051467801493No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT2170.11748909029875797No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT2140.11586481716098712No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA2120.11478196840247323No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2110.11424054402321628No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC2070.11207484650618849No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC2070.11207484650618849No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA2050.11099199774767458No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG2050.11099199774767458No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA2040.11045057336841763No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGTGAGACGAGAAAT2040.11045057336841763No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT2030.10990914898916068No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA1980.10720202709287593No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA1970.10666060271361899No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT1960.10611917833436205No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1950.10557775395510509No Hit
CCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT1940.10503632957584814No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC1920.10395348081733424No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT1880.10178778330030644No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG1880.10178778330030644No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC1850.1001635101625356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT151.2283942E-4145.06
AGCACGC1150.0138.695661
GCACGCA1200.0132.916672
CACGCAC1250.0127.63
ACGTGCA301.0981239E-7120.8333362
GAAACAC458.367351E-11112.777794
CGCACTT1500.0106.333335
ACGCACT1550.0102.903234
CACGTGC700.093.214291
AAACACG554.129106E-1092.272725
ACACGTG259.374546E-487.07
AAGCGGT259.374546E-487.01
CAGCCTT353.4298446E-582.857145
GTAGTAA2900.077.51
CGTGCCT406.64882E-572.53
ACGTGCC406.64882E-572.52
CGAGCCT300.00193323572.54
ACGAGCC300.00193323572.53
TAACAAG3300.070.303035
TACAAGA1050.069.0476158
Produced by FastQC (version 0.11.5)