FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_E10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_E10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111812
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT3040.2718849497370586No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1850.1654563016491969No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT1840.16456194326190393No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT1830.16366758487461097No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1800.1609845097127321No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA1730.15472400100168138No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT1700.15204092583980253No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA1650.14756913390333776No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1610.14399170035416592No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA1590.14220298357958003No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC1540.13773119164311523No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG1390.12431581583372088No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG1360.12163274067184202No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT1330.11894966550996317No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA1330.11894966550996317No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG1310.11716094873537725No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA1290.11537223196079133No Hit
GTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTC1290.11537223196079133No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA1250.1117947984116195No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC1240.11090044002432654No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA1220.10911172324974062No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC1190.10642864808786177No Hit
GTCCCATCCCCATCCTTTCAGTTTTAGACATCGTTCGAGAGTACCACCTT1180.10553428970056881No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT1170.10463993131327586No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA1160.1037455729259829No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1140.10195685615139699No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT1130.10106249776410403No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT100.007082501145.07
CACGCAC202.1222513E-6145.03
GCACGCA202.1222513E-6145.02
CGGTGCA100.007082501145.02
AGCACGC202.1222513E-6145.01
ACAACAG100.007082501145.01
GAAACAC301.0952499E-7120.8333364
ACGAGCC256.43959E-6116.02
CGAGCCT256.43959E-6116.03
GTAACAA1650.0114.242434
GAGCATC203.8569834E-4108.757
AAACACG352.7456008E-7103.571435
TAACAAG2000.094.255
TAGTAAC2050.091.9512252
CTTTAAG259.364797E-487.08
CACGTGC259.364797E-487.01
ACAAGAG2250.083.777787
CGCACTT353.423912E-582.857145
AGTAACA2450.076.9387743
TAGAAAC502.2928489E-672.52
Produced by FastQC (version 0.11.5)