FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_D6_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_D6_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216780
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5950.2744718147430575No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC4740.2186548574591752No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4390.2025094565919365No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT4300.19835778208321803No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC4230.19512870190977027No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA3920.18082848971307316No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3590.16560568318110527No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA2930.1351600701171695No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA2850.13146969277608636No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA2780.1282406126026386No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC2760.12731801826736783No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2740.12639542393209707No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA2570.11855337208229541No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC2520.11624688624411847No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG2520.11624688624411847No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2510.11578558907648308No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT2510.11578558907648308No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2500.11532429190884769No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT2460.11347910323830611No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT2320.10702094289141065No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT2320.10702094289141065No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT2270.10471445705323369No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT2270.10471445705323369No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC2250.10379186271796291No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTTTGG2230.10286926838269214No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC2190.10102407971215056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG100.007087059145.07
CTAACTT100.007087059145.02
TCTAACT100.007087059145.01
GAAACCG100.007087059145.0145
CGGTGCC202.1268042E-6145.02
AACACGT100.007087059145.06
AGCACGC700.0134.642851
CACGCAC800.0117.81253
GCACGCA800.0117.81252
ACGTGCA256.453367E-6115.9999852
CGTGCCT203.8619284E-4108.753
ACGTGCC203.8619284E-4108.752
CACGTGC501.9463187E-10101.499991
GAAACAC607.2759576E-1296.6666644
AAACACG607.2759576E-1296.6666645
ACAACGC554.1472958E-1092.2727368
CAACGCA554.1472958E-1092.2727369
AGCGTTT259.3767623E-486.999998
GCGTTTT259.3767623E-486.999999
CGCACTT1300.072.55
Produced by FastQC (version 0.11.5)