FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_D4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_D4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208354
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC14540.6978507732032982No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG13880.6661739155475777No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG7860.3772425775363084No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7360.35324495810015644No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA7030.3374065292722962No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA6690.3210881480557129No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA6610.3172485289459286No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA5490.2634938614089482No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA5370.25773443274427177No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA5350.2567745279668257No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC5340.25629457557810265No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4830.23181700375322767No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC4670.22413776553365908No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT4610.22125805120132083No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG4340.20829933670579878No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA4190.2011000508749532No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT4110.1972604317651689No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC3830.1838217648809238No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT3790.18190195532603165No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA3700.1775823838275243No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT3630.17422271710646303No Hit
AAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3450.16558357410944832No Hit
CACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA3380.16222390738838707No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA3350.16078405022221795No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT3250.15598452633498758No Hit
GAAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG3140.15070505005903415No Hit
GCACAGCTCTGGGAACTGGGAAAAGTGCTTCGGAAGGCTGGGCTGTCCGC3110.14926519289286505No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC3100.148785240504142No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3080.14782533572669593No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3060.14686543094924984No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT2990.14350576422818856No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2930.14062604989585034No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT2830.13582652600861994No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG2790.1339067164537278No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA2780.13342676406500475No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2760.13246685928755866No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA2730.13102700212138954No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT2720.13054704973266654No Hit
GTAGTAACAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA2710.13006709734394348No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT2580.12382771629054398No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT2530.12142795434692878No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC2500.11998809718075966No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC2480.11902819240331358No Hit
GTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCTTGGAAGCCAGA2480.11902819240331358No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2470.11854824001459056No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT2440.11710838284842144No Hit
CCTTATAAGAGACAAGCAGAGGGAGATGTAACATGCAGGCACACAACAGG2410.11566852568225233No Hit
GATATAGCCACAGCCAAGGCATGCTGGCGGCCGCCAGAAGCTGGAAGAGG2370.11374871612736016No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2310.11086900179502193No Hit
ATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCTTGCTT2300.1103890494062989No Hit
CACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA2290.10990909701757585No Hit
GTCCTACTGCAGCCTGTTGCCAAGGAGCTCCCCACAGACATGGAGCTCTC2260.10846923985140675No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2260.10846923985140675No Hit
CACTAAAGGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCAC2240.10750933507396067No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2190.10510957313034548No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT2160.10366971596417636No Hit
AGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA2160.10366971596417636No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA2160.10366971596417636No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA2150.10318976357545331No Hit
CCCTACAGCCACTTCTTCAGAAATTGAACTTGCTCTAAAATCCACTTCAT2140.10270981118673027No Hit
CTCCCACTCAGTCCTCAAAGATAGCAGGCCCTAGTCCTGGAGGCTGTAAA2130.10222985879800725No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA2120.10174990640928419No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA2120.10174990640928419No Hit
CCCTCTCTACCTTGCATGGTCTCTGCCCAGGACGCACCTCTGCCTAAGGG2110.10126995402056115No Hit
CCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGTGACAGGC2110.10126995402056115No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGT100.007086863145.0145
TATGCGA151.2286188E-4145.0145
GCCGTCC100.007086863145.03
GCGGTGC950.0137.368411
TAGTAAC2650.0134.056612
AGCACGC4200.0129.464281
ACGCACT4350.0126.6666644
CACGCAC4500.0122.4444353
CGCACTT4500.0122.4444355
CGGTGCA900.0120.8333362
GCACGCA4600.0119.7826162
GTAGTAA3150.0117.380951
AGTAACA3250.0109.307693
GCACTTC3000.0108.756
TAACAAG3300.0107.651515
GCACTTG2650.0106.698126
GGTGCAC900.0104.722223
TAGAAAC1950.0104.102572
GTAACAA3450.0102.971014
ACACGTG1000.0101.57
Produced by FastQC (version 0.11.5)