FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_D3_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_D3_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202653
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG20941.0332933635327382No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA17390.8581170769739407No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC16970.8373919951838859No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC16260.8023567378721264No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA16110.7949549229471066No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG16090.7939680142904373No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA16060.7924876513054334No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT13040.6434644441483718No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGG10840.5349044919147508No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT8470.41795581609944094No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA7960.3927896453543743No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC7920.39081582804103565No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG7020.34640493849091797No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC6690.3301209456558748No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA6140.30298095759746957No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5950.2936053253591114No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG5910.2916315080457728No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5860.28916423640409966No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA5810.2866969647624264No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG5410.2669587916290408No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC5370.2649849743157022No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG5180.25560934207734404No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG5090.25116825312233226No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG4870.24031225789897015No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4450.21958717610891526No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4370.2156395414822381No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT4260.21021154387055704No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG4250.20971808954222243No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4040.19935554864719496No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC4020.19836863999052567No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT4000.1973817313338564No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA3900.19244718805050998No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3630.17912392118547468No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA3480.17172210626045506No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT3420.1687613802904472No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3390.1672810173054433No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA3390.1672810173054433No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG3380.16678756297710864No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA3330.16432029133543544No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3290.16234647402209687No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC3230.15938574805208905No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTC3210.15839883939541977No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA3110.15346429611207335No Hit
CCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGT3050.1505035701420655No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG3040.15001011581373086No Hit
ATGCTAAGTCAGTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGA3030.14951666148539622No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC3010.14852975282872694No Hit
GTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCTAAGAG2980.147049389843723No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATG2920.14408866387371516No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC2890.14260830088871124No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2870.14162139223204195No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2870.14162139223204195No Hit
ATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT2830.1396475749187034No Hit
CTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA2820.13915412059036875No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA2760.1361933946203609No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCACT2750.13569994029202628No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2670.13175230566534915No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2650.13076539700867987No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2630.12977848835201058No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2600.12829812536700666No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2600.12829812536700666No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC2550.12583085372533345No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2550.12583085372533345No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT2530.12484394506866417No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT2520.12435049074032954No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA2480.12237667342699096No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATT2480.12237667342699096No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT2470.12188321909865632No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2410.11892249312864848No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAA2300.11349449551696741No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC2280.11250758686029816No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2240.11053376954695958No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT2230.11004031521862492No Hit
GCTTCAACCCGATGATTGTCGAACTTGCAGAAAAAGCAATGAAAGAGTAT2210.10905340656195567No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2180.10757304357695173No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC2090.10313195462193997No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATACTAGAGTCC2080.10263850029360531No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA2080.10263850029360531No Hit
GCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2080.10263850029360531No Hit
GTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2060.10165159163693603No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2050.1011581373086014No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG2030.10017122865193212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCT100.0070867203145.0145
AGCAAGG100.0070867203145.05
GTGTAAT100.0070867203145.01
GTTCCTA306.475602E-10145.01
CTTGTAG404.796675E-9108.751
ATTATTA203.8615614E-4108.751
CCTAGGT352.75375E-7103.571434
GAGCGAA1300.094.807691
CTAGGTA406.099781E-790.6255
TTCCTAG406.099781E-790.6252
AGGGTGT801.8189894E-1281.56256
ACAAGGG1000.079.753
AAGCAGG12150.072.7983557
TAGGTAG502.299601E-672.56
TAGAAAC2300.072.52
TAAGATC300.00193350872.53
AGCAGGA300.00193350872.58
CAAAAGC10650.070.798124
CAAGGGT1050.069.0476154
AAGGGTG955.456968E-1268.684215
Produced by FastQC (version 0.11.5)