FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_D1_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_D1_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196778
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGAGTTCAGTTTCAGATCTGTTCAATCTGTGATGTGTATTTAA2310.11739117177733283No Hit
GTACTGGGATTATAGGCGTTAGTCACCCTCCCAGCCCCTCTCCTCTCCTC1990.10112919127138195No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTA100.0070865653145.09
TTGCTCG100.0070865653145.0145
TATGCGG100.0070865653145.06
GAGCAAA2700.075.185191
AGCGAAA1350.069.814821
GCGAAAG1500.062.8333322
CGAAAGC1600.058.906253
GCACGTC450.00962771948.333332145
GCAAAAG4500.043.53
CACAGCG700.001054571141.428577
CAAAAGC4800.040.781254
GAGCGAA750.001480488838.6666641
AGCAAAA5350.036.5887872
GTACAAG800.002032648836.251
TATAGGA1251.2599405E-534.84
CTTTCGC1051.8483229E-434.5238088
TAGAAAC2603.6379788E-1233.4615362
ACTACTA900.003621205732.22222145
TACAGTG1402.7355698E-531.0714285
CTAAACA1453.476311E-530.04
Produced by FastQC (version 0.11.5)