FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_D12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_D12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136099
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5220.3835443316997186No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA3930.28876038765898354No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA2840.20867162874084305No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA2750.2020587954356755No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT2620.19250692510598902No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2560.18809836956921064No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC2440.17928125849565388No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2220.16311655486079987No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2130.1565037215556323No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC2110.15503420304337284No Hit
ATGTTATATTGATCTACTGTTGTTTGTTTCAGCACTGTTTTGGCTTCATT2090.15356468453111338No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT2010.14768661048207554No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT1980.14548233271368638No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT1950.14327805494529716No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA1930.14180853643303773No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC1880.13813474015238908No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1810.13299142535948097No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1810.13299142535948097No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1810.13299142535948097No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG1720.1263785920543134No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA1710.12564383279818367No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA1690.12417431428592422No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT1660.12197003651753502No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG1610.11829624023688638No Hit
ATTCAATACATCAAGAATTGAAAAGAACCCTTCATTAAGGATGAAGTGGG1590.11682672172462694No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1580.1160919624684972No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA1570.11535720321236746No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT1510.11094864767558908No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG1500.11021388841945938No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA1470.10800961065107018No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA1450.10654009213881073No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT1440.105805332882681No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT1430.10507057362655126No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA1420.10433581437042153No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT1390.10213153660203235No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT1390.10213153660203235No Hit
CCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT1380.10139677734590262No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC1370.10066201808977288No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1370.10066201808977288No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG151.2276889E-4145.01
GAAACAC450.0145.04
AAACACG500.0130.55
CACGCAC850.0119.4117663
GCACGCA850.0119.4117662
AGCACGC850.0119.4117661
GTACTGA203.8588027E-4108.752
ACCCACA203.8588027E-4108.759
ACACGAG501.9281288E-10101.57
AACACGA501.9281288E-10101.56
CGCACTT1100.098.863645
ACGCACT1150.094.565224
TAGAAAC800.090.6252
ACGTGCA259.3692E-487.02
CACCCAC259.3692E-487.08
AACACGT259.3692E-487.01
CACGAGC608.221832E-1084.5833368
GTTTAAC353.4265904E-582.857151
GTAGTAA2150.074.186041
ACAACGC300.001932137172.58
Produced by FastQC (version 0.11.5)