FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_D10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_D10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173294
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT5840.3369995498978614No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3460.19966069223400695No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA3380.1950442600436253No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA3150.181772017496278No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3080.17773263932969405No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG2880.16619155885373987No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2730.15753574849677426No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2660.1534963703301903No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2460.14195528985423617No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2430.14022412778284302No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT2430.14022412778284302No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC2400.13849296571144992No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA2380.1373388576638545No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA2150.12406661511650721No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT2100.12118134499751866No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2090.12060429097372097No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA2070.11945018292612555No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2070.11945018292612555No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC2030.1171419668309347No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG1960.11310258866435077No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT1930.11137142659295764No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT1910.11021731854536222No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC1890.10906321049776681No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT1880.10848615647396909No Hit
GATCTGGACGTAGCCTTGGGCAGACCAAAATGCACGGGGAAAATACCCTC1870.10790910245017139No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT1860.10733204842637367No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT1800.10386972428358743No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG1780.10271561623599201No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT1780.10271561623599201No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA1770.1021385622121943No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA1770.1021385622121943No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT1770.1021385622121943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG151.2282644E-4145.01
AGCACGC550.0131.818191
GTAACAA2300.0122.9347844
GAAACAC650.0122.692314
CACGCAC600.0120.8333363
GCACGCA600.0120.8333362
AACAATC203.8606054E-4108.757
CAACGCA203.8606054E-4108.759
AAACACG750.0106.333335
AACACGT301.5957112E-596.6666646
TAACAAG3050.095.081975
AACACGA554.129106E-1092.2727366
ACGCACT800.090.6254
CGCACTT800.090.6255
TAGTAAC3250.089.2307742
CAATCAC259.3735586E-487.09
ACGAGCC259.3735586E-487.09
GTAAGAG259.3735586E-487.02
GGTAAGA259.3735586E-487.01
ACACGAG608.240022E-1084.5833367
Produced by FastQC (version 0.11.5)