FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_C9_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_C9_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164502
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT5010.3045555677134625No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3920.23829497513708042No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3780.2297844403107561No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3280.1993896730738836No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2850.17325017325017325No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA2840.1726422779054358No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC2820.1714264872159609No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC2670.16230805704489915No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2620.15926858032121188No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA2610.15866068497647445No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC2580.1568369989422621No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2520.15318962687383741No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA2390.14528698739225054No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT2370.14407119670277566No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA2320.1410317199790884No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA2160.1313053944632892No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT2150.13069749911855175No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT2110.12826591773960194No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA2100.1276580223948645No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT2050.12461854567117725No Hit
TTCTAATGGTATGCTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTC1970.11975538291327764No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC1960.11914748756854021No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT1960.11914748756854021No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT1950.11853959222380275No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT1940.11793169687906531No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT1910.11610801084485295No Hit
CTCCTAGACTCGGCACTAAACCCCTCCAATTGGGCATTTCCAAATACATA1900.1155001155001155No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC1880.1142843248106406No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC1860.1130685341211657No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT1820.1106369527422159No Hit
ATACAAGGATATGACATGACTAAAGCTTGTTTCAAGGGAGACAGAGTAAA1810.11002905739747844No Hit
GAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAG1810.11002905739747844No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG1800.109421162052741No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGATGAAGAA1780.10820537136326609No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA1780.10820537136326609No Hit
CCATTAGAAAGATATAATGAAGAAACAAGAGCAAAATTAAGAAAGCTGAA1770.10759747601852863No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT1770.10759747601852863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC500.0145.01
ACAACGC100.007085516145.08
CACGCAC550.0131.818193
GCACGCA550.0131.818192
GAAACAC256.448703E-6116.04
ACGCACT650.0111.538474
CGCACTT650.0111.538475
TAGAAAC301.5954698E-596.6666642
GCGGAGC259.372711E-487.04
AGCGGAG259.372711E-487.03
GCACTTT1300.083.653856
GAAGCGG353.4287288E-582.857141
AAACACG406.6466644E-572.55
CAGAAGC406.6466644E-572.52
CACGTGC350.003561210462.1428571
GGTCTGC350.003561210462.1428578
TTAAGGT350.003561210462.1428574
CGTGCCT350.003561210462.1428573
TAAGGTC350.003561210462.1428575
ACGTGCC350.003561210462.1428572
Produced by FastQC (version 0.11.5)