FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_C8_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_C8_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168791
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4830.2861526977149256No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3340.19787784893744334No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC3280.19432315704036351No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2910.1724025570083713No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2610.1546290975229722No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2310.13685563803757309No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2190.12974625424341346No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2180.12915380559390016No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2170.12856135694438686No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2150.12737645964536023No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2150.12737645964536023No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG2120.1255991136968203No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC1980.11730483260363408No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC1960.11611993530460747No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA1960.11611993530460747No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT1960.11611993530460747No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT1870.11078789745898775No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1840.10901055151044782No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT1790.10604830826288132No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC1750.1036785136648281No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG1730.10249361636580151No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT1710.1013087190667749No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCA100.007085677145.02
CACGCAC458.367351E-11112.777783
GCACGCA458.367351E-11112.777782
AGCACGC458.367351E-11112.777781
GTAACAA1550.0107.580654
GCAGAAG352.7517308E-7103.571431
GAAACAC651.4551915E-1189.230774
ACGCACT608.240022E-1084.5833364
CGCACTT608.240022E-1084.5833365
AACACGT353.4289857E-582.857146
CAGAAGC451.2284327E-680.555562
AAACACG755.4569682E-1177.3333365
TAACAAG2250.074.111115
TAGAAAC851.6916601E-1068.235292
TAGTAAC2450.068.0612262
ACACGTG451.19099546E-464.444447
TGCGATA350.003561369162.142857145
ACAAGAG2750.060.636367
AGTAACA2750.060.636363
ACGTGCA553.2113277E-452.7272722
Produced by FastQC (version 0.11.5)