FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_C7_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_C7_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184891
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC9560.5170614037459909No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG6970.37697886863070673No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA6150.3326284134976824No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA5400.29206397282723334No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4910.26556187158920663No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG4810.2601532794998134No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG4000.21634368357572842No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA3770.203903921770124No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC3630.19633189284497354No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT3590.19416845600921626No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3530.1909233007555803No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA3400.18389213103936916No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3380.1828104126214905No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3220.17415666527846135No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3220.17415666527846135No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT3200.17307494686058272No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3090.1671254955622502No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA3060.16550291793543223No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT3060.16550291793543223No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC3050.16496205872649292No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC3040.16442119951755357No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT2990.16171690347285697No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2930.15847174821922105No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA2910.1573900298013424No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG2870.15522659296558514No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA2780.15035886008513125No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2730.14765456404043464No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT2730.14765456404043464No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2700.14603198641361667No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT2660.14386854957785938No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT2620.14170511274210212No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2610.14116425353316278No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2570.1390008166974055No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA2530.1368373798616482No Hit
ACCCAATGGTGTTCTCGCAACTCTACGGTATCCTGCAGCAGGAAACGAAT2520.1362965206527089No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2510.13575566144376958No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA2480.1341330838169516No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2450.13251050619013366No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2420.1308879285633157No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2420.1308879285633157No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA2380.1287244917275584No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2350.12710191410074043No Hit
TCCTTCGACTGTCCTTGCAGCTGGGATAAGAGTCCCTGACTCTGGTTATG2350.12710191410074043No Hit
GGCATGATGTGTCCCCCATGGCCATTGAAGAGTTGATTTGAACGATGACG2330.1260201956828618No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC2280.1233158996381652No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC2240.12115246280240789No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA2190.1184481667577113No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2180.11790730754877198No Hit
GCACAGCTCTGGGAACTGGGAAAAGTGCTTCGGAAGGCTGGGCTGTCCGC2170.11736644833983266No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA2160.11682558913089333No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA2150.11628472992195403No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA2140.1157438707130147No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT2020.10925356020574284No Hit
GAAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2000.10817184178786421No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA1990.10763098257892487No Hit
CTCTTGACCTGTTTGGCTTTAAAATCTGGGAGAGAGACACATCACTGCCA1980.10709012336998557No Hit
ATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCTTGCTT1910.1033041089074103No Hit
GCCGTATAGTGGATGCTGTCATCGAACAAGTCAAGGCCGCTGGAGCACTG1900.102763249698471No Hit
GATATAGCCACAGCCAAGGCATGCTGGCGGCCGCCAGAAGCTGGAAGAGG1880.10168153128059235No Hit
GCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGCATATCCTTCGACTG1860.1005998128627137No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGAC100.0070862197145.02
CCCCGTT403.274181E-11126.8759
GTAGTAA2000.0119.6251
TAACAAG2150.0111.2790765
TAGTAAC2250.0106.3333362
AAACACG1050.0103.5714345
GCGGTGC700.0103.571431
GCCCCCG501.9463187E-10101.57
CCCCCGT501.9463187E-10101.58
AACACGT501.9463187E-10101.56
AGCACGC2450.0100.612241
AGTAACA2400.099.68753
GCACGCA2550.096.666672
ACGCACT2800.088.035714
CGCACTT2800.088.035715
CACGCAC2950.083.559323
CACTTGA1700.081.029417
ACACGTG651.5552359E-978.076937
GTAACAA3200.077.031254
GCACTTG1900.072.56
Produced by FastQC (version 0.11.5)