000000000-J3T7H_l01_n02_C5_AR.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-J3T7H_l01_n02_C5_AR.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	166692
Sequences flagged as poor quality	0
Sequence length	151
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT	369	0.2213663523144482	No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC	318	0.19077100280757325	No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT	318	0.19077100280757325	No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT	300	0.17997264415808797	No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT	286	0.17157392076404387	No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT	285	0.17097401195018358	No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTATT	248	0.14877738583735273	No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT	244	0.14637775058191155	No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT	241	0.14457802414033066	No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT	238	0.14277829769874978	No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG	228	0.13677920956014686	No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT	223	0.13377966549084538	No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT	222	0.1331797566769851	No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT	221	0.13257984786312482	No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA	215	0.12898039497996305	No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC	203	0.12178148921363954	No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC	191	0.114582583447316	No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA	181	0.10858349530871308	No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA	178	0.10678376886713219	No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA	177	0.1061838600532719	No Hit
GTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTC	177	0.1061838600532719	No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA	177	0.1061838600532719	No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG	176	0.10558395123941161	No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC	175	0.10498404242555132	No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC	171	0.10258440717011014	No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC	171	0.10258440717011014	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATAAGG	25	3.6976417E-8	145.0	1
GGAGACG	10	0.0070855995	144.99998	145
CGGAGCG	10	0.0070855995	144.99998	4
AAGCGGA	20	2.1253454E-6	144.99998	1
GGAGCGT	10	0.0070855995	144.99998	5
CACGCAC	45	0.0	128.88889	3
GCACGCA	45	0.0	128.88889	2
GTAACAA	135	0.0	128.88889	4
GAAACAC	30	1.097651E-7	120.833336	4
AGCACGC	50	1.8189894E-12	116.0	1
ACAACAG	20	3.860345E-4	108.74999	1
CAGCCTT	20	3.860345E-4	108.74999	5
CAACGCA	20	3.860345E-4	108.74999	9
AGCGGAG	30	1.5955316E-5	96.666664	2
AAACACG	40	6.094979E-7	90.62499	5
ACGCACT	65	1.4551915E-11	89.230774	4
CGCACTT	65	1.4551915E-11	89.230774	5
CAGAGCG	25	9.3729293E-4	87.0	2
AGAGCGT	25	9.3729293E-4	87.0	3
TAGTAAC	200	0.0	87.0	2

Summary