FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_C4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_C4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230045
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA17140.7450716164228738No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC16530.7185550653133083No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA12190.5298963246321372No Hit
GTAGTAACAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA10750.46729987611119567No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT8750.38036036427655456No Hit
CACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA8540.37123171553391726No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG8320.36166836923210677No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA7910.34384576930600536No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA6870.29863722315199204No Hit
CACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA6770.29429024756025995No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG6730.29255145732356713No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT5960.2590797452672303No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG5550.2412571453411289No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5520.23995305266360928No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA5480.23821426242691648No Hit
ACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACC4980.2164793844682562No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA4980.2164793844682562No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA4670.20300376013388685No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG4560.1982220869829816No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG4560.1982220869829816No Hit
GTAGTAACAAGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT4550.19778738942380839No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT4500.19561390162794234No Hit
GCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCAT4370.1899628333586907No Hit
GTAGAAACACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT4280.18605055532613185No Hit
GTAGAAACACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT4150.18039948705688016No Hit
TTCTAGGATAGTTAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGG4060.17648720902432133No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3940.17127083831424286No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3890.16909735051837685No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT3880.16866265295920363No Hit
CAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA3720.16170749201243234No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3580.15562172618400746No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3490.15170944815144863No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT3460.150405355473929No Hit
GGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATT3390.14736247255971657No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3390.14736247255971657No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3350.14562368232302375No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC3280.1425807994088113No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3230.14040731161294528No Hit
GGATTAAGGCGACAGCGATTTCTAGGATAGTTAGTAGAATTAGAATTGTG3220.1399726140537721No Hit
AAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCG3200.13910321893542568No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT3090.13432154578452044No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT3010.1308439653111348No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA2950.12823577995609556No Hit
AGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACAT2920.12693168727857593No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2830.12301940924601708No Hit
AGTTAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAA2830.12301940924601708No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC2760.11997652633180464No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2750.11954182877263145No Hit
GCACAGCTCTGGGAACTGGGAAAAGTGCTTCGGAAGGCTGGGCTGTCCGC2740.11910713121345824No Hit
AGTAGAAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT2710.11780303853593861No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2700.1173683409767654No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2680.11649894585841901No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT2640.11476015562172617No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2630.11432545806255298No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2620.11389076050337978No Hit
CAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA2620.11389076050337978No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC2620.11389076050337978No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA2600.11302136538503336No Hit
GATATAGCCACAGCCAAGGCATGCTGGCGGCCGCCAGAAGCTGGAAGAGG2540.11041318002999413No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA2510.10910908735247453No Hit
GTAGAAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA2480.1078049946749549No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2470.1073702971157817No Hit
CTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCT2400.10432741420156927No Hit
TCTTTGGTCCATTGTCAAGAAAAGCCTTGCGAGGAACCCAATGGTGTTCT2400.10432741420156927No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA2360.10258862396487643No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2340.10171922884653004No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGT100.0070873387145.07
GTGCCCG100.0070873387145.04
AGCACGC3350.0132.014921
TAGTAAC4300.0123.0814062
GCACGCA3700.0119.5270162
AGTAACA4500.0117.6111153
TAACAAG4750.0111.421045
GCACTTG3350.0110.373136
GTAACAA4900.0109.489794
CACGCAC4050.0109.197533
CGCACTT4200.0107.02385
GTAGTAA4950.0106.91921
ACGCACT4150.0106.566264
GCACTTC1400.098.392856
CACTTGA3550.095.985917
AACAAGA5950.088.9495856
ACAAGAG6050.087.479337
CGCATCA259.377494E-487.0145
CGTTGAA259.377494E-487.01
GCGGTGC1350.085.925921
Produced by FastQC (version 0.11.5)