FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_C1_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_C1_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128369
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT7490.583474203273376No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT6310.4915516986188254No Hit
CATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTG5030.3918391511969401No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT4870.3793750827692044No Hit
GTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGC4830.37625906566227046No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4630.3606789801276009No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3630.282778552454253No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC3590.27966253534731905No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3330.25940842415224863No Hit
GTCAATTCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGC3300.25707141132204814No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3090.24071232151064512No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT3000.23370128302004378No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG2950.22980626163637638No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2940.22902725735964288No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT2740.21344717182497333No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT2710.2111101589947729No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA2710.2111101589947729No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA2690.20955215044130593No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT2660.2072151376111055No Hit
CTTCCTTCTTAATCCTTCCAGACTCGAAGTCAATTCTGGCATCAATCCGG2600.20254111195070462No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2500.19475106918336982No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG2460.19163505207643589No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2460.19163505207643589No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT2450.19085604779970242No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC2440.19007704352296895No Hit
CTACGGAATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGAC2390.18618202213930155No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG2360.1838450093091011No Hit
CGTACGTTCTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCG2360.1838450093091011No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT2320.18072899220216718No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA2090.16281189383729716No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2070.16125388528383022No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA2020.15735886390016282No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC2020.15735886390016282No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC2000.15580085534669585No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC1990.15502185106996239No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA1990.15502185106996239No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG1930.1503478254095615No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG1890.14723180830262758No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT1850.14411579119569365No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA1820.1417787783654932No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT1810.14099977408875974No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC1780.1386627612585593No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC1760.13710475270509234No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT1740.13554674415162538No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC1660.12931470993775757No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAGCAAGCGGGTCACT1650.12853570566102407No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG1640.1277567013842906No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC1640.1277567013842906No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA1610.12541968855409016No Hit
ATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA1530.11918765434022233No Hit
AACTCGAACCTGGGACCTTTGATCTTGGGGGGCTATACGAAGCAATTGAG1530.11918765434022233No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG1500.11685064151002189No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG1500.11685064151002189No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA1480.11529263295655494No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT1460.11373462440308797No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA1440.11217661584962102No Hit
CTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAAC1430.11139761157288752No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG1430.11139761157288752No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT1420.11061860729615405No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA1420.11061860729615405No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC1420.11061860729615405No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG1410.10983960301942058No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT1410.10983960301942058No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA1400.10906059874268709No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1400.10906059874268709No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA1380.10750259018922013No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA1370.10672358591248667No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT1360.10594458163575317No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA1330.10360756880555275No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT1310.10204956025208578No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG1300.10127055597535231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA100.007083715144.999989
CCTACTA100.007083715144.999985
CACCTAC100.007083715144.999983
TGAGAGT100.007083715144.999986
CCACCTA100.007083715144.999982
GAGCGAA501.8189894E-12116.01
GGATAAG203.8583E-4108.749991
GATAAGT259.3679834E-487.02
ACTTAGC259.3679834E-487.0145
TAACAGG259.3679834E-487.04
CTTAATC1050.082.857148
AGCGAAA1150.081.956531
TTAATCC1100.079.090919
GCGAAAG1250.075.42
TAGAAAC1650.074.696972
GTAGAAA1650.074.696971
GAGCAAA4250.073.3529361
CTAGTGC300.001931886572.500019
CGAAAGC1300.072.53
AAGGTCG702.7866918E-972.59
Produced by FastQC (version 0.11.5)