FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_C12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_C12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140313
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT7570.5395080997484196No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA4870.3470811685303571No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC4690.334252706449153No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA3640.25942001097546197No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA3010.21452039369124742No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA2950.21024423966417935No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT2880.20525539329926665No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG2530.18031116147470297No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC2520.1795984691368583No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2260.16106846835289673No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT2230.1589303913393627No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2220.15821769900151803No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2160.15394154497444998No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT2150.15322885263660532No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT2150.15322885263660532No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2150.15322885263660532No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG2120.15109077562307127No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT2100.14966539094738193No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC2080.14824000627169256No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1990.1418257752310905No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA1880.13398615951479906No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1770.12614654379850762No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC1770.12614654379850762No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACTTAGACTCTGCCTT1770.12614654379850762No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC1740.1240084667849736No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA1730.12329577444712891No Hit
AGACAATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC1690.12044500509575022No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT1680.11973231275790554No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT1660.11830692808221618No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA1640.11688154340652684No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA1540.10975462002808009No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT1500.10690385067670138No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA1500.10690385067670138No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA1490.1061911583388567No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA1490.1061911583388567No Hit
CTGTATATCAGTTAAGCATCTTTTGTTTTTTATCCATTCCAAGGCAGAGT1490.1061911583388567No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA1480.10547846600101202No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA1430.10191500431178864No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC1410.10048961963609929No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG1410.10048961963609929No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGG100.007084413145.05
TAGCAGT100.007084413145.05
ATATCGT100.007084413145.01
TTACCCG100.007084413145.04
AACACGT202.1241613E-6145.01
ACGCACT750.0116.000014
CACGCAC750.0116.000013
GCACGCA750.0116.000012
CGCACTT750.0116.000015
AGCACGC750.0116.000011
ACACGTG256.445369E-6116.02
GAAGCGG256.445369E-6116.01
GAAACAC203.8590576E-4108.754
AAACACG203.8590576E-4108.755
CGGAGCG203.8590576E-4108.755
GTAACAA2200.088.977274
TAGAAAC259.3698164E-487.02
CAAGAGG1100.085.6818168
AGCGGAG353.426966E-582.857143
TAACAAG2450.079.897965
Produced by FastQC (version 0.11.5)