FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_C11_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_C11_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182794
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC7780.42561572042846046No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA6650.36379749882381257No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA6570.35942098755976676No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5090.2784555291749182No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT4680.25602590894668315No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA4590.25110233377463154No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA4550.2489140781426086No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG4480.24508463078656847No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT4410.24125518343052835No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG3980.21773143538628184No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA3580.1958488790660525No Hit
CTATAATTGTGAGTCGGGGTCTTAACCTGACTAATTTATCAGCTAGAAGA3420.18709585653796076No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG3350.1832664091819206No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA3240.17724870619385757No Hit
GTGTATGTAGTGGTTTAGAGCTGGAATTTGGCATAACTGGGTTTTTATTT3150.17232513102180597No Hit
GTCTTAACCTGACTAATTTATCAGCTAGAAGAGATAAAGCCAAGGGATGC3060.16740155584975436No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3060.16740155584975436No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3060.16740155584975436No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2980.1630250445857085No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT2930.16028972504567984No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT2860.15646027768963972No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2740.1498955107935709No Hit
CTCTTCTTCTTTTCCAAAAGTGTTTTGATGCACTTATGAAGGATAGATTC2730.14934844688556517No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA2730.14934844688556517No Hit
CTCTAGTTGTGTCCTAATACATCTGTTCTGGATTCCCATGCTACTGGTAA2720.14880138297755943No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2710.14825431906955372No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2700.14770725516154798No Hit
GATAATGGCCTCTGTCAAAGCAAAGAATAGTTGTAGAGACACAAACTGGG2680.1466131273455365No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2540.13895423263345624No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2500.1367659770014333No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2460.13457772136941037No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC2440.13348359355339892No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC2390.13074827401337025No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2380.1302012101053645No Hit
GTGCTACAGATTAGCATTTACCATTGTTTTAGGTGCTTTACATGTAACAA2360.12910708228935305No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2330.12746589056533583No Hit
GTCCTCATTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAG2230.1219952514852785No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC2170.11871286803724411No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2160.11816580412923837No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT2150.11761874022123264No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA2080.1137892928651925No Hit
AATCTATCCTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGA2040.11160103723316958No Hit
GCATTAAAGGTATCCATTGATGATTCTGTCCATTCATTTAACACTACACA2010.10995984550915237No Hit
GATTAGCATTTACCATTGTTTTAGGTGCTTTACATGTAACAACCTATTTA2000.10941278160114663No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2000.10941278160114663No Hit
TTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAGTTGGACT1990.10886571769314092No Hit
AGCTTAGAGTATGCTCTAGTTGTGTCCTAATACATCTGTTCTGGATTCCC1980.1083186537851352No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT1970.10777158987712945No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA1950.10667746206111797No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA1950.10667746206111797No Hit
TCCTTATACAAGAGTAAACTTATTCTTATCAACCTGATGGTGGGTCAAAG1940.10613039815311225No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA1890.10339507861308359No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC1870.1023009507970721No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT1860.10175388688906638No Hit
ACCCAATGGTGTTCTCGCAACTCTACGGTATCCTGCAGCAGGAAACGAAT1860.10175388688906638No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC1860.10175388688906638No Hit
GGCATGATGTGTCCCCCATGGCCATTGAAGAGTTGATTTGAACGATGACG1840.10065975907305492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG550.0145.01
AAGAGCG151.228374E-4145.07
GACTCGT100.0070861555145.06
AGCACGC2100.0134.642851
CACGCAC2150.0131.511633
GCACGCA2150.0131.511632
GCACTTG1750.0107.714296
ACGCACT2800.0100.982144
CGCACTT2900.097.55
TAGTAAC2050.091.951222
GAGCGCC259.3743904E-487.09
AACACGT850.085.294126
ACAACGC608.240022E-1084.5833368
GCGGTGC353.4297496E-582.857141
GGCGCCA353.4297496E-582.85714145
GTAGTAA2300.081.956521
AAGCGGA1000.079.752
CACTTGA2150.077.5581367
AAACACG1500.077.333335
TAACAAG2600.072.55
Produced by FastQC (version 0.11.5)