FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_C10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_C10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249646
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT9710.3889507542680436No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA6940.2779936389928138No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA6360.2547607412095527No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC5310.21270118487778694No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA5190.20789437843987085No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT5070.20308757200195476No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG4690.18786601828188715No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4690.18786601828188715No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4650.18626374946924845No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT4290.17184333015550018No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC3780.1514144027943568No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT3710.1486104323722391No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA3350.13419001305849082No Hit
GTGTAACTGTTATCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAA3280.1313860426363731No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA3200.1281815050110957No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG3160.126579236198457No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA3090.1237752657763393No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAGCA3070.12297413137001995No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT3020.12097129535422159No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT3020.12097129535422159No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2960.11856789213526353No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2900.11616448891630549No Hit
GTATCAGTCTCCACGTTTAATTTGACAAAAGGTCTTTTTGCTGTGTAACT2880.11536335450998614No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT2860.1145622201036668No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC2820.1129599512910281No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2810.11255938408786842No Hit
TTCTATGGTTTTATTGCTGGCAAATCCGCATGTGCATTCCTCAGTGTGTT2810.11255938408786842No Hit
GGAAAATACAGGGCTTAACTTCCCTTATAGACAGATGGAGCAAGAAACAT2790.11175824968154907No Hit
GACACATACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGC2760.11055654807207005No Hit
GTGTTTTACTCTTCCTGTTGGAAATATTTCTTTTATTATTCGGCCCTCTC2740.1097554136657507No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2730.10935484646259101No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT2720.10895427925943135No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA2710.10855371205627168No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT2600.10414747282151526No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT2570.10294577121203624No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC2540.10174406960255722No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG2530.10134350239939754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATG100.007087697145.04
GTGATAC100.007087697145.03
GGAAGAC100.007087697145.04
CATAAGG100.007087697145.01
GAGCAGA100.007087697145.01
ACGAGCC151.2289084E-4145.09
GTGGTAA100.007087697145.03
GAAACAC700.0113.928574
GTAACAA2350.0111.0638354
CACGCAC700.0103.571433
GCACGCA700.0103.571432
CGGAGCA352.7556234E-7103.571432
AGCACGC650.0100.384611
AAACACG800.099.68755
CAACGCA406.1039464E-790.6259
GCGGAGC406.1039464E-790.6251
CAGCCTT259.378439E-487.04
ACGTGCC259.378439E-487.09
ACGTGCA502.2617314E-887.02
ACCCACA353.4322147E-582.857149
Produced by FastQC (version 0.11.5)