FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B9_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B9_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203574
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT7840.3851179423698508No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA5680.27901401947203475No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC5490.26968080403194905No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT5280.2593651448613281No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT5180.2544529262086514No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA4940.2426636014422274No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT4530.22252350496625306No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA4270.2097517364692937No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA4250.20876929273875838No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4200.20631318341242005No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT4130.2028746303555464No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC4010.19697996797233439No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT4000.1964887461070667No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC3720.18273453387957203No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC3590.1763486496310924No Hit
GAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAG3560.17487498403528937No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC3480.17094520911314803No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC3230.1586646624814564No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT3210.15768221875092106No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGATGAAGAA3160.1552261094245827No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT3070.15080511263717372No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT3040.1493314470413707No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC2980.1463841158497647No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA2950.1449104502539617No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA2880.14147189719708805No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC2790.13705090040967904No Hit
GGTTTATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCA2640.12968257243066406No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA2550.12526157564325505No Hit
TTATTATTGTCATTCCTATTGCCATGTCTGCTGATTCATTTACTCCAGCA2520.12378791004745203No Hit
TTCTAATGCTGTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCAC2480.12182302258638136No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC2470.1213318007211137No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT2460.12084057885584604No Hit
TTCTAATGGTATGCTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTC2420.11887569139477537No Hit
GTATAGCACCGGTCTTATTCTCCAATAAAATAGCCAGACTGGGAAAAGGA2400.11789324766424004No Hit
GTATGGAAGGGATAAACGACTTTTACCGAACATGCAAATTATTGGGAATA2400.11789324766424004No Hit
CTCTATTGGTTATTGGTATTACTTTGTATTTTCCCATGCTGGCATTGCTT2390.11740202579897235No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT2380.11691080393370469No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT2360.11592836020316936No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT2360.11592836020316936No Hit
ATACAAGGATATGACATGACTAAAGCTTGTTTCAAGGGAGACAGAGTAAA2270.11150736341576037No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT2260.1110161415504927No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA2260.1110161415504927No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA2250.11052491968522501No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA2190.10757758849361902No Hit
CTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAGAGATTTATA2180.10708636662835136No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT2140.1051214791672807No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC2130.10463025730201303No Hit
GATTTATAGAAGTTGGAATAACAAAAGGATTGGCCGATGATTACTTTTGG2080.1021741479756747No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT2060.10119170424513935No Hit
CTCTTGGGTCTGTACTACTAAATTCGAAAGTTACAACTGTTACAACATCA2050.10070048237987168No Hit
CCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAGAGATTTATAGA2050.10070048237987168No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAG100.007086744145.02
TAACGGA100.007086744145.05
CACGCAC253.700734E-8144.999983
GCACGCA253.700734E-8144.999982
AGCACGC253.700734E-8144.999981
ACAACGC256.4524156E-6115.9999858
CAACGCA256.4524156E-6115.9999859
GCGGTGC259.375935E-486.999991
GAGGTGG259.375935E-486.999999
ATATAGG259.375935E-486.999993
GTAGTAA1950.085.512811
ACACGAG607.9995516E-872.57
ATCGAAT300.001933520372.53
ACGCACT502.2996373E-672.499994
CGCACTT502.2996373E-672.499995
AAACACG651.3924728E-766.923085
AACACGA651.3924728E-766.923086
AACAACG451.19158365E-464.444447
GAAGCGG350.00356242562.1428571
GAAACAC702.3252323E-762.1428574
Produced by FastQC (version 0.11.5)