FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B8_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B8_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207191
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4430.21381237602019393No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT4430.21381237602019393No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC3980.19209328590527583No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3760.18147506407131586No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT3410.1645824384263795No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT3310.15975597395639773No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT3280.1583080346154032No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2850.13755423739448142No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG2810.1356236516064887No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC2810.1356236516064887No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG2620.12645336911352328No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2590.12500542977252874No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2510.1211442581965433No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA2480.11969631885554875No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA2440.11776573306755603No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTGCTAGTTTTCCTTCT2440.11776573306755603No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2430.11728308662055784No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT2410.11631779372656148No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT2410.11631779372656148No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT2360.11390456149157058No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT2350.11342191504457239No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAA2270.10956074346858695No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2250.1085954505745906No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2240.1081128041275924No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCAGCTGG2220.10714751123359606No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2210.10666486478659787No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC2200.1061822183395997No Hit
TCCAAATACATAGTGCATCAAGCATTTAGTAAATATTACTCTTAGTGCTT2190.10569957189260151No Hit
GTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTC2180.10521692544560332No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2160.10425163255160697No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA2110.10183840031661606No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG2100.10135575386961788No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTT100.007086833145.04
GGAGCGT100.007086833145.04
CTGCCTT100.007086833145.05
CGGAGCG100.007086833145.03
CACGCAC500.0130.53
GCACGCA500.0130.52
AGCACGC500.0130.51
GTAACAA2450.0124.285714
CAACGCA352.75395E-7103.571439
AAACACG800.099.68755
CAGCCTT301.5964528E-596.6666645
GAAACAC850.093.8235244
TAGTAAC3250.093.692312
TAACAAG3350.090.895525
ACGTGCA406.100254E-790.6252
GAGCATC406.100254E-790.6256
AGAAGCG259.3761674E-487.02
ACAAGAG3550.085.774657
ACAACAG608.258212E-1084.5833361
ACGCACT801.8189894E-1281.56254
Produced by FastQC (version 0.11.5)