FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B6_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B6_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73936
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2410.325957584938325No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA1770.23939623458125947No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA1310.1771802640121186No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT1220.16500757411815625No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC1190.1609500108201688No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA1180.15959748972083965No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT1130.15283488422419392No Hit
ATTGTATTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC1110.1501298420255356No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT1080.14607227872754816No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1060.14336723652888986No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1030.1393096732309024No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT1020.13795715213157325No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1020.13795715213157325No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1020.13795715213157325No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG1000.13525210993291495No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC980.13254706773425667No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT920.12443194113828177No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC910.1230794200389526No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTTTGG910.1230794200389526No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG910.1230794200389526No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA890.12037437784029432No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTT870.11766933564163601No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT850.1149642934429777No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT840.11361177234364857No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT810.10955420904566111No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT800.10820168794633195No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC800.10820168794633195No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC800.10820168794633195No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC790.10684916684700282No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT780.10549664574767367No Hit
CCCCATGGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT770.10414412464834451No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA770.10414412464834451No Hit
CACGTGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA750.1014390824496862No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT740.10008656135035707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG151.2254364E-4145.01
CACGCAC600.0145.03
GCACGCA600.0145.02
GAAACAC306.4210326E-10145.04
AGCACGC600.0145.01
GCGGTGC100.0070776753144.999981
ACGTGCA100.0070776753144.999982
ACTACAT100.0070776753144.999985
AAACACG351.877197E-9124.285725
ACGCACT800.0117.812494
CGCACTT800.0117.812495
ACAACGC203.8517502E-4108.749998
AACAACG203.8517502E-4108.749997
CAACGCA203.8517502E-4108.749999
ATATTGA353.4162105E-582.857156
AGCGGAG300.001928631272.53
TATTGAT406.6224704E-572.499997
GTGTAAA406.6224704E-572.499991
GTAGTAA955.456968E-1268.684211
TAGTAAC955.456968E-1268.684212
Produced by FastQC (version 0.11.5)