FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B5_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B5_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89447
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2520.2817310809753262No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT1760.19676456449070398No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1450.16210716960881863No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1440.16098918912875781No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1420.15875322816863618No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT1340.14980938432814964No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA1290.14421948192784553No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC1240.13862957952754146No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA1240.13862957952754146No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT1180.13192169664717654No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG1170.13080371616711572No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA1110.12409583328675082No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG1100.12297785280669No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC1080.12074189184656835No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1080.12074189184656835No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA1070.11962391136650753No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA1070.11962391136650753No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC1070.11962391136650753No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1050.1173879504063859No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC1030.11515198944626427No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1010.11291602848614263No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA1000.11179804800608181No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT980.10956208704596018No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT950.10620814560577771No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT930.10397218464565608No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT910.10173622368553445No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA900.10061824320547363No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG100.007080144144.999981
ACGAGCC100.007080144144.999982
CGAGCCT100.007080144144.999983
CCTTATG100.007080144144.999984
GTAACAA850.0127.941174
GAAACAC403.274181E-11126.874994
AACACGA351.882654E-9124.285716
AAACACG458.367351E-11112.777785
ACACGAG404.7657522E-9108.749997
CACGCAC203.8544263E-4108.749993
GCACGCA203.8544263E-4108.749992
AGCACGC203.8544263E-4108.749991
ACAACAG203.8544263E-4108.749991
ACGTGCA203.8544263E-4108.749992
TAACAAG1100.098.863635
CACGTGC301.591466E-596.6666641
TAGAAAC554.110916E-1092.272722
GTCATAA259.358611E-487.01
CACGAGC502.2449967E-887.08
TAGTAAC1500.077.333332
Produced by FastQC (version 0.11.5)