FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45986
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC3760.817640151350411No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2870.6241029878658723No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA2500.5436437176531989No Hit
GTAGTAACAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA2400.5218979689470709No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA2330.5066759448527812No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG2310.5023267951115556No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA2270.49362849562910455No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT2240.4871047710172661No Hit
GCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCAT1600.34793197929804726No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT1490.32401165572130647No Hit
ACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACC1490.32401165572130647No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT1480.32183708085069374No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG1370.29791675727395295No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA1280.2783455834384378No Hit
AAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCG1210.2631235593441482No Hit
CACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA1160.2522506849910843No Hit
AGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACAT1080.23485408602618188No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA1080.23485408602618188No Hit
CTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCT990.21528291219066673No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA970.21093376244944112No Hit
CAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA970.21093376244944112No Hit
CAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA960.20875918757882833No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA950.20658461270821557No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT890.19353716348453878No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC870.18918801374331318No Hit
CACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA820.1783151393902492No Hit
GCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATT800.17396598964902363No Hit
TCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATACAACTACGCAAA780.16961683990779802No Hit
GTAGAAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA780.16961683990779802No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT760.16526769016657245No Hit
CCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCT720.15656939068412126No Hit
AGTAGAAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT720.15656939068412126No Hit
ATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGTCCATTGT720.15656939068412126No Hit
AACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGC710.15439481581350845No Hit
CCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTA700.15222024094289568No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG670.14569651633105726No Hit
CCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTA670.14569651633105726No Hit
TTCTAGGATAGTTAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGG670.14569651633105726No Hit
CTCTTCTTCTTTTCCAAAAGTGTTTTGATGCACTTATGAAGGATAGATTC640.1391727917192189No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT630.13699821684860608No Hit
ATATACAACTACGCAAAGGCCCCAACGTTGTAGGCCCCTACGGGCTACTA620.13482364197799332No Hit
CACGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGC620.13482364197799332No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT620.13482364197799332No Hit
CACGTGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGC620.13482364197799332No Hit
TCTTTGGTCCATTGTCAAGAAAAGCCTTGCGAGGAACCCAATGGTGTTCT620.13482364197799332No Hit
CCGCTAACATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCC610.1326490671073805No Hit
CCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACA580.12612534249554214No Hit
GTAGAAACACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT550.11960161788370374No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA550.11960161788370374No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC550.11960161788370374No Hit
GTCCTCATTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAG540.11742704301309094No Hit
CTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTAC520.11307789327186535No Hit
AGAAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC520.11307789327186535No Hit
GTAGTAACAAGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT500.10872874353063976No Hit
GTAGAAACACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT490.10655416866002697No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT480.10437959378941417No Hit
GGATTAAGGCGACAGCGATTTCTAGGATAGTTAGTAGAATTAGAATTGTG480.10437959378941417No Hit
GTAGTAACAAGAGCATATTTCACCTCCGCTACCATAATCATCGCTATCCC470.10220501891880138No Hit
CTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGTCCATTGTCA470.10220501891880138No Hit
AAGCAGAGCCCGGTAATCGCATAAAACTTAAAACTTTACAGTCAGAGGTT460.10003044404818859No Hit
GGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGTTAGTAGAATTAGAA460.10003044404818859No Hit
AGCACAGCCTTCAGATGCCAGAAGGGCAGAGGGAATCCCACACGGGCTGG460.10003044404818859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA600.0145.02
CGGTGCG100.0070690196145.02
GCCAGTA100.0070690196145.08
CGGAGCC151.2224412E-4145.03
CTTACGA100.0070690196145.01
AACACGT100.0070690196145.06
CGCACTT700.0134.642855
CACGCAC650.0133.846163
GCACTTG500.0130.56
ACGCACT850.0119.411764
AGTAACA750.0116.000013
AGCACGC750.0116.000011
GTAGTAA700.0113.928571
TAGTAAC700.0113.928572
TAACAAG800.0108.755
CACTTGA600.0108.757
ACTTCAG301.5831945E-596.6666648
CAATAGA301.5831945E-596.6666641
CAGAGCA700.093.214294
ACGTGCA406.024511E-790.6252
Produced by FastQC (version 0.11.5)