FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B3_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B3_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216340
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG22861.0566700563927152No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC19980.9235462697605621No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA19920.9207728575390589No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA19480.9004345012480356No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA19210.8879541462512711No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC18590.8592955532957383No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG16280.7525191827678653No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT15490.7160025885180734No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT10280.4751779606175464No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGG10270.47471572524729594No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC10050.4645465471017842No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA9430.43588795414625126No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC8200.3790330036054359No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA7350.3397429971341407No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG7050.3258759360266248No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC6510.30091522603309606No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG6500.30045299066284553No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA6470.2990662845520939No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC6020.27826569289082No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG5780.26717204400480726No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG5740.2653231025238051No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA5360.24775815845428495No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA5080.23481556808727005No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT4820.22279744846075622No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG4690.2167883886474993No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA4690.2167883886474993No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4620.2135527410557456No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA4620.2135527410557456No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA4490.20754368124248868No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4320.19968567994822964No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT4300.19876120920772855No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT4300.19876120920772855No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG4290.19829897383747802No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC4150.1918276786539706No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTC4100.18951650180271795No Hit
GTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCTAAGAG4000.18489414810021262No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA3800.175649440695202No Hit
ATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT3690.17056485162244617No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC3640.1682536747711935No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3570.16501802717943978No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG3540.16363132106868816No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATG3490.1613201442174355No Hit
CCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGT3470.16039567347693445No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC3460.15993343810668392No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3340.15438661366367756No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC3290.15207543681242489No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT3220.14883978922067118No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG3210.14837755385042065No Hit
ATGCTAAGTCAGTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGA3190.14745308310991956No Hit
CTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3160.146066376999168No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3160.146066376999168No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT3060.14144402329666267No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATT3050.1409817879264121No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3040.1405195525561616No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3010.13913284644541No Hit
GCTTCAACCCGATGATTGTCGAACTTGCAGAAAAAGCAATGAAAGAGTAT2990.13820837570490893No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2920.13497272811315522No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT2910.1345104927429047No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCACT2880.13312378663215307No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2830.13081260978090042No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2820.1303503744106499No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT2800.12942590367014886No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC2700.12480354996764352No Hit
GTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2700.12480354996764352No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAA2590.11971896089488768No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCC2540.11740778404363503No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2530.11694554867338448No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG2520.11648331330313395No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT2480.11463437182213183No Hit
GGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGA2430.11232319497087918No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC2410.11139872423037811No Hit
CTTAGGGACAAACTCGAACCTGGGACCTTTGATCTTGGGGGGCTATACGA2400.11093648886012758No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2400.11093648886012758No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC2360.10908754737912546No Hit
ATCTATAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT2360.10908754737912546No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2230.10307848756586854No Hit
AGTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGC2230.10307848756586854No Hit
GTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTT2220.102616252195618No Hit
CCACAATTGGAAGGATTTTCAGCAGAGTCAAGAAAACTGCTCCTTGTTGT2210.10215401682536747No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATACTAGAGTCC2200.10169178145511694No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2200.10169178145511694No Hit
CTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG2200.10169178145511694No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT2200.10169178145511694No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA2170.10030507534436535No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG100.007087048145.01
GTTCCTA403.274181E-11126.8751
CCTAGGT351.895387E-9124.285714
CTAGGTA351.895387E-9124.285715
GCAGGGG550.0118.636379
GAGCGAA1100.0105.454551
AGGGTGT406.1011633E-790.6256
AAACTAT3750.077.3333366
CAAAAGC9850.075.076144
TAGAAAC2300.072.52
GCAGGGT300.001933684572.57
AAGCAGG11950.071.589967
TTTTTGG3800.070.592111
TTTAAAC4250.069.941183
TTCCTAG651.392873E-766.923072
GGTGTTT554.0508603E-665.9090968
CAAGGGT554.0508603E-665.9090964
TAAACTA4600.064.619575
GCAGGTC5000.063.89
TAGGTAG808.047209E-963.43756
Produced by FastQC (version 0.11.5)