FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B1_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B1_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161544
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3680.22780171346506217No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3310.20489773683949886No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC3020.18694597137621946No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2950.182612786609221No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2930.18137473381865002No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2870.17766057544693706No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2830.17518446986579508No Hit
TTCTTATACAGTTCCCCCTAAAACAGAAATTGAATGTACTTACTTGAGCA2150.1330906749863814No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT1800.1114247511513891No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1730.10709156638439063No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC406.6462955E-572.500013
CAATTCG502.2974818E-672.59
CGATACC300.001932794672.5145
CAGTACG554.0462164E-665.909099
CGAAAGC606.779872E-660.4166683
TCGACAC606.779872E-660.4166683
CAAAAGC4250.054.5882384
AAGCAGG4750.051.8947377
CGACACT701.6896058E-551.7857134
AGCGAAA701.6896058E-551.7857131
GACAGTA858.877887E-751.176477
GGCCGTG858.877887E-751.176478
GCCGTGT858.877887E-751.176479
TAAGGCC1004.6704372E-850.755
GAGCAAA4650.049.8924751
GTATTAA2050.049.5121961
CAGCGTG450.00962462248.3333327
ATTCGAC604.9326924E-448.3333321
AGGGTGT450.00962462248.3333326
AGGCCGT901.3166318E-648.3333327
Produced by FastQC (version 0.11.5)