FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158700
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT3550.2236925015752993No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3280.20667926906112163No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA3040.19155639571518587No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG2790.17580340264650285No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT2760.17391304347826086No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA2660.16761184625078765No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC2660.16761184625078765No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2600.1638311279143037No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2490.15689981096408318No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2450.1543793320730939No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT2410.1518588531821046No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG2370.14933837429111532No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA2310.14555765595463138No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2230.1405166981726528No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA2210.13925645872715817No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2190.13799621928166353No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2190.13799621928166353No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2160.13610586011342157No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT2130.1342155009451796No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2110.13295526149968492No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG1990.12539382482671707No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT1970.12413358538122243No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA1940.12224322621298048No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT1900.11972274732199117No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG1870.1178323881537492No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG1840.11594202898550725No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG1830.11531190926275992No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT1810.11405166981726528No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT1780.11216131064902331No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC1750.11027095148078135No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1710.10775047258979205No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT1670.10522999369880277No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT1660.10459987397605545No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA1640.10333963453056082No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC1640.10333963453056082No Hit
GTATATGCTTTTCCTTCTTCGTCAAGAAAATTCATGTCACTTATTACATA1640.10333963453056082No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA1630.10270951480781348No Hit
TGTCATATCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGT1620.10207939508506615No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA1620.10207939508506615No Hit
CTCCTAATTAGCCCTCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC1610.10144927536231885No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG1600.10081915563957151No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC1590.10018903591682418No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1590.10018903591682418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG306.475602E-10145.01
ACAACAG202.125029E-6144.999981
CGGTGCG202.125029E-6144.999985
GGTGCGT202.125029E-6144.999986
AAACACG301.0973963E-7120.8333365
GTAACAA1200.0120.8333364
AGCACGC650.0111.538461
TAGAAAC352.7509668E-7103.571432
CACGCAC700.0103.571433
GCACGCA700.0103.571432
GAAACAC352.7509668E-7103.571434
GTGCGTT301.5952957E-596.6666647
GAGCATC554.129106E-1092.272727
ACGCACT800.090.624994
ACGTGCA259.372099E-487.02
GCAGAAG259.372099E-487.01
CGCACTT850.085.294115
TAGTAAC1700.085.294112
CAGAGCA608.240022E-1084.5833365
CAGAAGC300.001932732472.52
Produced by FastQC (version 0.11.5)