FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_B10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_B10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206088
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA15640.7588991110593534No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT7740.37556771864446253No Hit
GATAATGGCCTCTGTCAAAGCAAAGAATAGTTGTAGAGACACAAACTGGG6390.3100617212064749No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC5900.2862854702845386No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA5780.2804627149567175No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT5240.25426031598152243No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA5200.2523193975389154No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT5090.24698187182174608No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA4900.2377625092193626No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG4880.23679204999805908No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4750.23048406505958619No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA4330.21010442141221225No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT4200.20379643647373938No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA4120.1999145995885253No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA3740.1814758743837584No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA3660.1775940374985443No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3630.17613834866658903No Hit
CTCTTCTTCTTTTCCAAAAGTGTTTTGATGCACTTATGAAGGATAGATTC3570.17322697100267845No Hit
GGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCT3480.16885990450681262No Hit
GTCCTCATTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAG3280.1591553122937774No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT3250.15769962346182215No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA3210.1557587050192151No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG3180.1543030161872598No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3100.15042117930204574No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA3030.14702457202748342No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA2920.14168704631031404No Hit
CTATAATTGTGAGTCGGGGTCTTAACCTGACTAATTTATCAGCTAGAAGA2870.13926089825705523No Hit
CTTATAAGGACATTCATAATTGCACATAGAACTCACCTGAATAATCCAGA2860.13877566864640348No Hit
GTCTTAACCTGACTAATTTATCAGCTAGAAGAGATAAAGCCAAGGGATGC2840.13780520942509997No Hit
GTGTATGTAGTGGTTTAGAGCTGGAATTTGGCATAACTGGGTTTTTATTT2830.1373199798144482No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2830.1373199798144482No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA2780.13489383176118938No Hit
TTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAGTTGGACT2750.13343814292923412No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT2730.1324676837079306No Hit
GAATAGTTGTAGAGACACAAACTGGGCCCTTGAAGCTGTGACTAACATTT2720.13198245409727882No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA2700.1310119948759753No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT2670.12955630604402005No Hit
ATATATGCCAATAAGAAAACATTCTTTGCAAAAGTCTTCCATGCAAATAA2550.1237335507161989No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2540.12324832110554715No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT2490.12082217305228835No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2330.11305849928186018No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG2320.11257326967120843No Hit
GTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATA2320.11257326967120843No Hit
GCATTAAAGGTATCCATTGATGATTCTGTCCATTCATTTAACACTACACA2210.10723574395403905No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA2200.1067505143433873No Hit
CAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGAAGAAAAG2170.105294825511432No Hit
GAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGAAACATCAAATT2150.10432436629012849No Hit
AGTAGAAACAACGCACTTTGGGATAGTAACTGATGTTTGTGTTTGGTGAG2150.10432436629012849No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2140.10383913667947674No Hit
GTTTTATCGTATGTCCCAGTCCAATATTTGCACTCTTAGCTGCAAAACTT2140.10383913667947674No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2100.10189821823686969No Hit
GATTAGCATTTACCATTGTTTTAGGTGCTTTACATGTAACAACCTATTTA2070.1004425294049144No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGCC100.0070868065145.03
GCACGCA2250.0125.666672
AGCACGC2250.0125.666671
GTAGTAA4600.0108.751
AGTAACA4650.0107.580653
TAGTAAC4750.0105.315792
CACGCAC2700.0104.722213
TAACAAG5050.099.05945
CGCACTT3250.089.230775
ACGCACT3300.087.8787844
AAGAGCG702.910383E-1182.857149
CGGTGCA1050.082.857142
AACAAGA6300.080.555566
GCGGTGC1350.080.555551
AAGAGCC4400.075.7954569
GCACTTC1250.075.46
ACAAGAG6950.073.021587
GAAGCGG903.6379788E-1272.51
AAACACG1800.072.55
ACGAGCT300.00193355472.59
Produced by FastQC (version 0.11.5)